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A practical technique for isolation and cryopreservation of tench (Tinca tinca) (Cyprinidae, Teleostei) early stages of germ cells (GC), including spermatogonia and spermatocytes, is reported for the...
Oil with higher a content of ω-3 polyunsaturated fatty acids (PUFA) was administered for 4 weeks to experimental germ-free piglets and from 21 days of age the piglets were inoculated perorally with pr...
Gnotobiotic piglets (n = 10) were hand-reared in conventional germ-free facilities. Piglet body temperatures were measured with rectal and non-contact infrared thermometry (NIFT) on the lower eyelid, ...
采用超声波处理小麦胚芽球蛋白以提高其功能性质。研究了超声波处理对小麦胚芽球蛋白理化和功能性质的影响。结果表明:经超声波处理后,小麦胚芽球蛋白的巯基和二硫键含量、紫外光谱和荧光光谱均发生了显著的变化。随着超声波功率的增加,小麦胚芽球蛋白的疏水性、起泡性、起泡稳定性、乳化性和乳化稳定性都降低;当超声波功率大于900?W时,由于小麦胚芽球蛋白重新伸展,疏水基团暴露增多,引起疏水性、起泡性、起泡稳定性、乳...
麦胚具有快速酸败变质的特性,为提高麦胚稳定性,利用研发的麦胚在线微波稳定化处理工艺和装备,选取不同微波功率和处理时间等工艺参数进行试验,研究不同条件对麦胚稳定化效果的影响。结果表明,采用微波功率2.8kW、时间4min处理的麦胚,在30d加速贮藏后,脂肪酶相对酶活17.63%,过氧化值3.81mmol/kg,酸价14.65mg/g,VE损失率5.29%,氮溶解指数51.10%,含水率3.72%,脂...
Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 ...
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Avian primordial germ cells (PGCs) show a unique migration pathway during early development. As soon as the blood vessels have formed, PGCs enter the circulatory system, and migrate to the gonadal pri...
In order to develop the technique for in vitro culture of chicken primordial germ cells obtained from circulating embryonic blood vessels (cPGCs), participation of feeder cells and some of growth fact...
Developmental Genetic Analysis of the Avian Primordial Germ Cells and the Applications for Production of Chimeric Chickens.
Avian primordial germ cells (PGCs) arise from the epiblast and circulate temporarily via the blood vascular system in their migration route to the gonadal anlage. There are egg-to-egg variations in th...
A genetically selected broiler line (42L) developed at the Pennsylvania State University, PA, USA was used in the present study. In Experiment 1, embryonic blood was collected from chick embryos at ...
The migratory ability of germ cells collected from gonads at different stages of embryonic development was investigated in the domestic chicken. In Experiment 1, primordial germ cells (PGCs) were col...
The effect of ultraviolet (UV) irradiation on the ability of primordial germ cells (PGCs) to migrate toward the gonadal ridge was investigated in the domestic chicken. When PGCs were irradiated with ...

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