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旨在建立鸭坦布苏病毒（DTMUV）的特异性血清学诊断方法。本研究以纯化的单克隆抗体1A5为固相筛选分子，利用噬菌体展示技术鉴定DTMUV囊膜（E）蛋白的特异性抗原表位，并利用DNAStar分析抗原表位序列在DTMUV中保守性和与其他黄病毒的E蛋白相应位点的氨基酸序列相似性。结果成功筛选到DTMUV E蛋白的一个线性抗原表位87YAEYI91，该抗原表位在DTMUV中具有高度的保守性，无氨基酸位点差...
Epitope tagging has been widely
used for studies of protein structure and
function (8). The epitope tagging
process generally begins with a cDNA
encoding the protein of interest. A major
weakness...
Sequential IgE epitope analysis of a birch pollen allergen (Bet v1) and an apple allergen (Mal d1)
apple Bet v1 birch immunotherapy Mal d1 sequential IgE epitope
2010/11/25
Cross-reactivity in IgE epitopes and T cell epitopes has been reported between major birch pollen allergen (Bet v1) and major apple allergen (Mal d1). To treat people with birch pollinosis complicated...