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In vitro sperm-binding assay to distinguish differences in populations of human sperm or damage to sperm resulting from cryopreservation
Sperm quality fresh versus frozen semen
2009/2/18
Annually, >1.3 million men are members of couples seeking help because of infertility. Semen from many of these men contains reasonable numbers of motile and normal sperm, but for a subset of indivi...
An improved mouse sperm-oocyte plasmalemma binding assay: studies on characteristics of sperm binding in medium with or without glucose
Zona-free oocytes pronuclear zygotes in vitro fertilization
2009/2/18
Sperm binding to the oocyte plasmalemma is crucial to subsequent steps in fertilization. However, the usual in vitro assay for sperm-oocyte binding does not distinguish between nonspecific attachmen...
Sequence divergence within the sperm-specific polypeptide TCTE1 is correlated with species-specific differences in sperm binding to zona-intact eggs
Sperm egg binding zona pellucida evolution fitness
2009/2/17
The T-complex-associated testes-expressed (TCTE1) gene encodes a novel sperm cell-specific polypeptide (TCTE1) that is conserved across vertebrate species. TCTE1 is absolutely required for fertili...
Functional aspects of human sperm binding to the zona pellucida using the hemizona assay
Human zona pellucida hyperactivation infertility
2009/2/7
The hemizona assay (HZA) has facilitated investigations of sperm function in relation to zona pellucida binding. In this study, the authors examined: 1) the association between hyperactivated sperm ...