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Synchronous assay for human sperm capacitation and the acrosome reaction
human sperm capacitation acrosome reaction
2009/2/23
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
The meaning of sperm capacitation. A historical perspective
sperm capacitation historical perspective
2009/2/20
It should be recalled that sperm capacitation was originally defined in 1952 as some physiological changes of the spermatozoa in the female genital tract before they are capable of penetrating and fer...
Ca2+ Is Required for Mouse Sperm Capacitation and Fertilization In Vitro
capacitation acrosome reaction whiplash motility A23187 fertilization in vitro calcium
2009/2/19
The temporal involvement of Ca2+ during mouse sperm capacitation in vitro was examined by releasing sperm into Ca2+-free medium and then introducing Ca2+ (1.80 mM, final concentration) at 30 minute ...
In Vitro Sperm Capacitation and In Vitro Fertilization with Normal Development in the Rabbit
sperm capacitation in vitro fertilization embryo culture embryo transfer spermatozoa oocytes rabbit
2009/2/19
The objective was to obtain in vitro sperm capacitation and fertilization to approximate the normal in vivo processes. Rabbit oocytes from follicles, ovarian surfaces, and oviducts were fertilized...
Interaction between Ca2+, cyclic 3',5' adenosine monophosphate, the superoxide anion, and tyrosine phosphorylation pathways in the regulation of human sperm capacitation
Calmodulin phosphatase kinase
2009/2/17
In order to fertilize the egg, spermatozoa must go through the capacitation process where they experience Ca2+ uptake, increases in cyclic 3',5' adenosine monophosphate (cAMP) concentrations, supe...
Human sperm capacitation induced by biological fluids and progesterone, but not by NADH or NADPH, is associated with the production of superoxide anion
Spermatozoa MCLA reactive oxygen species sperm hyperactivation protein tyrosine
2009/2/17
Recent evidence indicated that human sperm capacitation is associated with an increased production of superoxide anion (O2.-). To further study the role and importance of O2.- in capacitation, we in...
Low levels of nitric oxide promote human sperm capacitation in vitro
NONOates reactive oxygen species catalase hydrogen peroxide peroxinitrite nitric oxide synthase
2009/2/13
The influence of nitric oxide on human sperm hyperactivation and capacitation, as well as its mechanism of action and its possible origin from spermatozoa were studied. Percoll-washed spermatozoa ...
Influence of bovine follicular and oviduct fluids on sperm capacitation in vitro
bovine follicular oviduct fluids sperm capacitation
2009/2/7
The ability of bovine follicular fluid (FF) and oviduct fluid (ODF) to capacitate sperm was determined. At concentrations of greater than 20%, both FF and ODF capacitated sperm within 4 hours, but a...
Lipid transfer activity in human follicular fluid：relation to human sperm capacitation
sperm capacitation acrosome reaction lipid transfer cholesteryl ester
2009/2/6
A potentially important event during sperm capacitation is the loss of sperm membrane cholesterol. Although the exact mechanisms mediating this loss are not known, albumin and high density lipopr...
Semenogelin, the main protein of semen coagulum, inhibits human sperm capacitation by interfering with the superoxide anion generated during this process
Protein tyrosine phosphorylation prostate-specific antigen
2008/12/29
Semenogelin (Sg), the major protein of the human semen coagulum, is present at high concentrations in seminal vesicle secretions. It is degraded by the prostate-specific antigen (PSA) to generate pe...
Mice Lacking Raf Kinase Inhibitor Protein-1 (RKIP-1) Have Altered Sperm Capacitation and Reduced Reproduction Rates With a Normal Response to Testicular Injury
PEBP testis
2008/12/24
Raf kinase inhibitor protein-1 (RKIP-1) belongs to the phosphatidyl ethanolamine–binding family of proteins (PEBP), which are highly conserved throughout evolution and widely expressed in tissues...
Annexin V Binding and Merocyanine Staining Fail to Detect Human Sperm Capacitation
FACScan membrane architecture phosphatidylserine translocation
2008/12/22
The signaling pathways that characterize the process of capacitation of human spermatozoa are still largely unknown. Modifications in the lipid architecture of the sperm plasma membrane have been de...
Inhibitors of Phosphoinositide 3-Kinase, LY294002 and Wortmannin, Affect Sperm Capacitation and Associated Phosphorylation of Proteins Differently：Ca2+-Dependent Divergences
Spermatozoa signal transduction Akt
2008/12/22
Sperm capacitation is regulated by multiple pathways that also control sperm motility and tyrosine (Tyr) phosphorylation of several sperm proteins. Among the reported pathways, phosphoinositide 3-ki...
Implication of Calmodulin-Dependent Phosphodiesterase Type 1 During Bovine Sperm Capacitation
Vinpocetine rolipram cyclic AMP in vitro fertilization motility
2008/12/18
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) h...
Activation of Protein Kinase A During Human Sperm Capacitation and Acrosome Reaction
Phosphodiesterases cyclic adenosine monophosphate calcium ionophore A23187 signal transduction
2008/12/15
Spermatozoa undergo a variety of changes during their life that are prerequisites to their maturation and ability to fertilize eggs. Mammalian sperm capacitation and acrosome reaction are regulated by...