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Polyamine profiles in rat testis, germ cells and Sertoli cells during testicular maturation
Polyamine profiles rat testis germ cells Sertoli cells
2009/2/23
Polyamine cellular concentrations (putrescine, spermidine and spermine) in the rat testis and testicular cell types were determined by fluorescence spectroscopy of their dansyl derivatives. A met...
Effect of germ cells on vectorial secretion of androgen binding protein and transferrin by immature rat Sertoli cells in vitro
germ cells vectorial secretion androgen binding protein transferrin immature rat Sertoli cells
2009/2/23
The influence of germ cells (greater than 85% pachytene spermatocytes) on vectorial secretion of androgen binding protein (ABP) and transferrin by immature rat Sertoli cells was investigated using t...
Secretion of androgen binding protein by Sertoli cells is influenced by contact with germ cells
androgen binding protein Sertoli cells germ cells
2009/2/20
Sertoli cells were cultured alone or with germ cells to evaluate the effect of the association with germ cells on the secretory activity of Sertoli cells. Secretion of androgen-binding protein, whic...
Squash preparation studies of germ cells in human fetal testes
testis preleptotene germ cells chromosomes
2009/2/20
Testes from human fetuses 21 to 41 weeks of gestation were investigated using a modified squash technique. At all of the gestational ages examined, 90 to 95% of the germ cells were in interphase. Pr...
Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid-treated mice as determined by flow cytometry
Spermatogenesis TUNEL
2009/2/18
Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by mo...
Evidence for cross-talk between Sertoli and germ cells using selected cathepsins as markers
cross-talk Sertoli germ cells
2009/2/18
To examine whether proteases are possibly involved in cellular migration and/or spermiation when developing germ cells translocate across the seminiferous epithelium during spermatogenesis, in sit...
Rat testicular germ cells and epididymal sperm contain active P450 aromatase
Estrogen testis epididymis
2009/2/17
Although testosterone is the principal sex steroid produced by the testis, estrogen is known to be produced by both Leydig and Sertoli cells during different developmental periods. Additionally, evide...
Spontaneous degeneration of testicular germ cells in congenitally athymic nude mice of four strains：a light microscopical observation
Spontaneous degeneration testicular germ cells
2009/2/17
Many hormonal studies have demonstrated that the presence of thymic factors affects testicular function. However, morphological studies on the testis in the absence of thymic factors are very limite...
P450 aromatase messenger ribonucleic acid expression in male rat germ cells: detection by reverse transcription-polymerase chain reaction amplification
Germ cells spermatids P450 aromatase estrogen
2009/2/17
We have previously demonstrated that cytochrome P450 aromatase (P450arom) protein, an estrogen-synthesizing enzyme, is present and active in germ cells of the adult mouse testis. To establish that P...
Computer tracking of germ cells in the cycle of the seminiferous epithelium and prediction of changes in cycle duration in animals commonly used in reproductive biology and toxicology
Testis spermatogenesis kinetics male germ cells toxicology modelling
2009/2/10
A computer program called Stages was written to aid the tracking of germ cells and stages forward and backward through time in the cycle of the seminiferous epithelium. The program incorporates the ...
Regulation of Sertoli cell myotubularin（rMTM）expression by germ cells in vitro
Sertoli cell myotubularin（rMTM） germ cells vitro
2008/12/26
Recent studies have shown that rat myotubularin (rMTM), the homolog of human myotubularin, which is a putative protein tyrosine phosphatase (PTP), is expressed by Sertoli cells in the rat testis. In a...
Dynamic subcellular distribution of the DAZL protein is confined to primate male germ cells
Dynamic subcellular distribution DAZL protein primate male germ cells
2008/12/25
We have examined the location of the DAZL protein in fetal and adult rodents and human specimens and found that there is a shift from a predominantly nuclear to a predominantly cytoplasmic distribut...
Epigenetics in Male Germ Cells
Epigenetics Male Germ Cells
2008/12/24
We provide an overview of epigenetic mechanisms comprising DNA methylation, histone modification, and RNA-associated silencing of gene expression by noncoding RNAs and subsequently focus attention...
Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats
Irradiation busulfan testis spermatogonial transplantation
2008/12/24
Testicular cell transplantation has been widely used to investigate the biology of spermatogonial stem cells, production of transgenic animals, and restoration of fertility in rodent models. O...
Depletion of Endogenous Germ Cells in Male Pigs and Goats in Preparation for Germ Cell Transplantation
Irradiation busulfan testis transgenesis large animals
2008/12/23
The efficiency of germ cell transplantation, the procedure of transferring germ cells from a donor male into the testes of recipient males, can be greatly increased by reduction of endogenous germ c...