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Genetic and environmental parameters estimation for milk traits in Slovenian dairy sheep using random regression model
dairy ewes test-day records orthogonal Legendre polynomials heritability additive genetic correlations
2015/6/24
(Co)variance components for daily milk yield, fat, and protein content in Slovenian dairy sheep were estimated with random regression model. Test-day records were collected by the ICAR A4 method. Anal...
The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds
sheep FABP4 gene polymorphism intramuscular fat
2015/6/9
The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality trait...
Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats
random amplified polymorphic DNA cattle sheep and goats typing
2015/2/5
n this study, 30, 15 and 1 strains of Pasteurella multocida and 9, 8 and 6 strains of Mannheimia haemolytica from cattle, sheep and goats isolated in Elazig province located in the East ...
多浪羊MHC-DRB3基因座的PCR-RFLP多态性分析Polymorphism Analysis of MHC-DRB3 Gene in Dolang Sheep with PCR-RFLP
多浪羊 MHC-DRB3 分子遗传多态性 PCR-RFLP
2008/1/10
摘要 主要组织相容性复合体(MHC)是由紧密连锁的高度多态的基因位点所组成的染色体上的一个遗传区域,它在动物机体的免疫系统中发挥着非常重要的作用。应用PCR-RFLP技术首次对多浪羊的MHC-DRB3的外显子2进行分子遗传多态性检测与分析。结果显示,多浪羊MHC-DRB3基因的外显子2在TaqⅠ、PstⅠ和HaeⅢ酶切位点存在多态,其酶切位点分别由2、2和6种共显性等位基因控制。综合3种酶切结果,...
利用微卫星标记进行凉山半细毛羊亲权鉴定的研究Microsatellite Markers for Paternity Testing of Liangshan Semi-fine Wool Sheep
凉山半细毛羊 微卫星标记 多重PCR 亲权鉴定
2008/1/6
摘要
经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物,并用Cervus 2.0分析。结果表明:当双亲未知时,累积排除概率为0.998666 ,在父系半同胞基础上进行母子亲权鉴定,累积排除概率为0.999994,置信度为95%,最终从8个候选父亲中找出215个后裔的父亲,又从147个候选母亲中找出它们的母亲,构建了一个混合家系系谱,为进行连锁分析、QTL定位...
摘要
以绵羊BMP15基因和BMPR-IB基因为候选基因,以湖羊、中国美利奴单胎品系、中国美利奴肉用和毛用多胎品系为研究对象,采用PCR-RFLP方法对候选基因进行单核苷酸多态性(SNP)位点检测和基因型分析,同时研究基因对绵羊产羔数的影响。对BMP15基因进行SNP检测,结果未发现多态性位点;对BMPR-IB基因进行多态性检测,结果发现了一个A746 G SNP位点。依据A746 G SNP位...
我国主要地方绵羊品种随机扩增多态DNA研究Studies of Random Amplified Polymorphic DNA(RAPD) of Main Indigenous Sheep Breeds in China
地方绵羊品种 引入品种 随机扩增多态DNA 遗传亲缘关系
2007/12/29
对蒙古羊、湖羊、滩羊、小尾寒羊、乌珠穆沁羊、藏绵羊、阿勒泰羊7个地方绵羊品种和无角陶赛特羊、德国美利奴羊、萨福克羊3个引入品种基因组DNA进行了RAPD分析。结果表明:(1)RAPD可作为一种有效的标记用于绵羊品种之间遗传亲缘关系的分析。(2)在所使用的43种随机引物中,有35种引物扩增出多态谱带,多态频率为66.24%,说明RAPD技术用于研究绵羊核DNA的遗传变异具有较高的检出率和灵敏度。(3...