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Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants
Plant gene editing Fluorescent seed selection Co-transformation Cas9 zCas9i pEC1.2 PcUBi4-2
2023/11/30
Multiplex CRISPR-Cas9-based genome editing is an efficient method for targeted disruption of gene function in plants. Use of CRISPR-Cas9 has increased rapidly in recent years and is becoming a routine...
GmANN, a glutathione S-transferase-interacting annexin, is involved in high temperature and humidity tolerance and seed vigor formation in transgenic Arabidopsis
Soybean Annexin Arabidopsis High temperature and humidity stress Vigor
2024/3/4
Plant annexins are proteins with various functions, such as binding calcium ions and negatively charging lipids. Although their importance in plant stress responses has been widely reported, their fun...
SMC5/6 Complex Subunit NSE4A is Involved in DNA Damage Repair and Seed Development in Arabidopsis
Seed Development DNA Damage NSE4A subunit SMC5/6 Complex
2024/3/4
The maintenance of genome integrity over cell divisions is critical for plant development and the correct transmission of genetic information to the progeny. A key factor involved in this process is t...
Overexpression of NHL6 affects seed production in transgenic Arabidopsis plants
Arabidopsis Development NHL6 Seedproduction
2024/3/8
In higher plants, NHL (NDR1/HIN1-like) genes play important roles in response to both biotic and abiotic stresses. However, their biological functions in plant growth and development are largely unkno...
Infl uence of sycamore seed stratifi cation length on their germination capacity
sycamore seed handling stratification
2015/5/5
Sycamore (Acer pseudoplatanus L.) seeds are frequently used in forest nurseries and therefore the knowledge of their proper treatment is desirable. Seed stratification recommended by the Czech standar...
Germination and emergence response of specifi c Douglas fi r seed lot to diff erent temperatures and prechilling duration
stratification germination capacity emergence rate
2015/5/5
One of the critical points of Douglas fir seedling production is to optimize the time of sowing and length
of pre-sowing treatment. Germination and emergence of Douglas fir seed lot of the Czech ori...
Testing of germination of spruce, pine and larch seed after 10 years from collection – Short Communication
germination capacity germination energy
2015/5/5
Germination capacity and germination energy are usually the most frequently used quantitative parameters of forest tree seed. With seed ageing both parameters decreased and the rate of the collapse is...
Characteristics and Effectiveness of Glasshouse Miniature Seed Orchards in Cryptomeria japonica D. Don
Cryptomeria japonica D.Don glasshouse miniature seed orchard pollen contamination
2009/8/28
We produced seeds of Cryptomeria japonica trees in a glasshouse miniature seed orchard to prevent pollen contamination. In the glasshouse, male flowers shed pollen from February 4 to March 27, 2002, w...
Effects of Snow Cover on Seed Survival and Seedling Distribution of Beech Protection from Seed Predation by Rodents
beech tree regeneration seed predation seedling bank snow cover
2009/8/24
Beech trees often dominate the natural forests in northeast part of Japan. This is partly attributable to heavy snow environment in these districts. Deep snow cover during winter season may contribute...