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[目的] 利用RNA 干扰（RNA interfering ,RNAi）技术构建和鉴定膜联蛋白A3（Annexin A3）的真核表达载体，为进一步研究
Annexin A3在肝细胞中的功能奠定基础。[方法] 人工合成3 对shRNA序列，退火后定向克隆到真核表达载体psiRNA-hH1neo上，采
用酶切和测序法鉴定。[结果] 质粒酶切及测序鉴定得到的产物与预期的目的基因一致。[结论] 成功构...
CELL DEATH DIFFERENTIATION IN BLACK HEADED RAMS SPERMATOZOA, USING FLUORESCENT LABELED ANNEXIN V
spermatozoa plasma membrane phosphatidylserine
2010/12/6
Double staining kit of Annexin V Cy3.18/6-CFDA was used to investigate the changes in phospholipide asymmetry after treating sperm cells with dexamethasone. The % of spermatozoa with registered transl...