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华中农业大学在植物Rubisco遗传改良方面取得新进展
华中农业大学 植物 Rubisco 遗传改良
2023/2/24
近日，华中农业大学生命科学技术学院、作物遗传改良全国重点实验林拥军教授团队联合英国利物浦大学刘鲁宁教授科研团队在利用细菌快速Rubisco优化高等植物光合作用领域取得新进展。相关研究结果以“Producing fast and active Rubisco in tobacco to enhance photosynthesis”为题在线发表在国际期刊The Plant Cell。
[目的] 氮素营养影响着水稻灌浆过程中核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco）和谷氨酰胺合成酶（GS）基因转录表达量，研究其变化动态及其与不同形态氮含量的关系，旨在为阐明氮素营养对光合效率和籽粒蛋白质积累的影响分子调控机理提供理论依据。[方法] 选用寒地粳稻穗数型高产品种和穗重型超级稻品种进行盆栽试验。施肥比例为N：P2O5：K2O=1：0.5：1，氮肥50%作为基肥，其余作分蘖肥和...
由中国科学院亚热带农业生态研究所副所长（主持工作）吴金水研究员领衔的农业生态过程方向研究团队近日在土壤微生物固碳关键酶RubisCO酶活性提取与测定方法研究方面取得了新进展。
黄瓜Rubisco活化酶基因CsRCA表达载体构建与遗传转化
黄瓜 核酮糖&ndash 15&ndas
2012/10/17
核酮糖–1,5–二磷酸羧化/加氧酶（Rubisco）是光合碳循环中的关键酶，为了探明其在植物体内的活化机制，用农杆菌介导法将Rubisco活化酶（RCA）基因CsRCA导入黄瓜，分别用PCR、real-time PCR和Western杂交法进行分子检测，分析转基因植株的表达量。酶切鉴定结果显示，CsRCA正向插入植物表达载体pCAMBIA1301的CaMV 35S启动子和NOS终止子之间，成功构建...
Endeavours of RuBisCO small subunit promoter as a tool of green tissue specific expression
constitutive expression rbcS promoter spatio-temporal expression
2014/2/25
Transcriptomics has the potential to rapidly increase our knowledge of spatial and temporal gene expression and contributes to the characterization of new promoters for research and development. The s...
芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析
芥菜 Rubisco小亚基 抗病性 叶绿素荧光 TuMV
2011/11/25
以耐病性叶用芥菜为材料,克隆和分析了芥菜Rubicso小亚基的基因序列,研究了芜菁花叶病毒(Turnip mosaic virus,TuMV)侵染对Rubicso小亚基基因表达的影响。结果显示:Rubicso小亚基基因全长为842bp,其开放阅读框(ORF)可编码181个氨基酸,其中包括2个α螺旋及4个β折叠二级结构,并含有一些高度保守的氨基酸残基。接种TuMV后,随着上部第3片真叶中外壳蛋白基因...
Rubisco活化酶(RCA)是一种可激活光合作用关键酶Rubisco的伴侣蛋白，可通过对Rubisco的活性调节决定植物的碳同化效率。为了研究玉米ZmRCA1的多态性，本研究参考GenBank中编码玉米Rubisco活化酶的ZmRCA1基因组序列设计引物对玉米微核心种质的95份自交系的ZmRCA1进行测序，获得长约1 680 bp的基因组序列。多态分析表明，在1 680 bp的区间内共发现22个...
［目的］研究玉米、水稻等木兰门作物RubisCO大亚基的分子适应性进化。［方法］以玉米、水稻等木兰门作物为研究对象，利用利用密码子替换模型和位点间取不同ω值的最大似然法模型对叶绿体rbcL基因进行分子适应性进化分析。［结果］RubisCO受到正选择作用，并鉴定出了6个正选择位点。［结论］该研究鉴定的6个正选择位点对于研究RubisCO大亚基催化活性和作物改良具有重要的指导意义。
水稻叶片自然衰老过程中Rubisco大亚基的含量变化
水稻 叶绿素b 突变体 衰老 光合放氧 1,5二磷酸核酮糖羧化酶/加氧酶
2009/9/23
以水稻低叶绿素b突变体及其野生型（镇恢249）为材料，研究了水稻第5叶叶片自然衰老过程中光合放氧、Rubisco大亚基含量变化以及对胰蛋白酶敏感性的变化，并通过喷施不同的抗氧化剂以及氧化剂研究其对Rubisco大亚基含量变化的影响。结果表明，叶片全展后随着衰老进程，突变体与野生型光合放氧速率、Rubisco大亚基相对含量呈下降趋势，抗氧化剂可以不同程度地缓解Rubisco大亚基含量的下降，而氧化剂...
Photosynthetic Control Factors in a Single Leaf of Sweet Potato, Ipomoea batatas Lam : 3. Estimation of in vivo rubisco activity from the CO2 exchange rate of a peeled leaf
CO2 exchange rate Ipomoea batatas Lam Leaf epidermis peeling Rubisco activity
2009/7/30
Rubisco (ribulose 1, 5-bisphosphate carboxylase/oxygenase) activity is the prime determinant for CO2 exchang rate (CER) at the beginning of the photosynthetic reaction, and hence the rubisco activity ...
Relationships between Photosynthetic Activity and the Amounts of Rubisco Activase and Rubisco in Rice Leaves from Emergence through Senescence
Aging Oryza sativa L Photosynthesis Rice Rubisco Rubisco activase Senescence
2009/7/27
Photosynthetic activity determined as O2 evolution (OER) and the amounts of Rubisco activase, Rubisco, total soluble protein and chlorophyll were investigated in the 10th leaf of rice, Oryza sativa L....
Correlation of Leaf Nitrogen,Chlorophyll and Rubisco Contents with Photosynthesis in a Supernodulating Soybean Genotype Sakukei 4
Chlorophyll Glycine max Leaf nitrogen Photosynthesis Rubisco Soybean Supernodulation
2009/7/24
Soybean requires more nitrogen (N) than gramineous crops because it accumulates a large amount of N in seeds, and its photosynthetic rate per leaf N is low. The supernodulating genotype Sakukei 4 has...
Gene Expression and Accumulation of Rubisco in Bundle Sheath and Mesophyll Cells during Leaf Development and Senescence in Rice,a C3 Plant
Bundle sheath C3 photosynthesis Chlorophyll fluorescence Immuno-localization In situ hybridization Leaf development Leaf senescence Mesophyll
2009/7/23
Gene expression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (rbcL) and small subunit (rbcS) in bundle sheath and mesophyll cells of rice, a C3 plant, was examined during...
Screening of High kcat Rubisco among Poaceae for Improvement of Photosynthetic CO2 Assimilation in Rice
kcat Km Oryza sativa L. Photosynthesis Rice Rubisco
2009/7/21
The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a major limitation of photosynthetic CO2 assimilation in C3 plants. In order to find useful Rubisco for improvement of phot...
The QTL Analysis of RuBisCO in Flag Leaves and Non-Structural Carbohydrates in Leaf Sheaths of Rice Using Chromosome Segment Substitution Lines and Backcross Progeny F2 Populations
Backcross progeny Chromosome segment substitution lines (CSSLs) Leaf sheath Non-structural carbohydrate (NSC) QTL Rice (Oryza sativa L.) RuBisCO
2009/7/21
In rice (Oryza sativa L.), the maintenance of high photosynthetic rate of flag leaves and the carbon remobilization from leaf sheaths after heading is a critical physiological component affecting the ...