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牦牛DGAT1基因多态性及其与乳质性状关联分析
牦牛 DGAT1基因 多态性 乳品质性状
2017/11/9
检测牦牛DGAT1基因多态性，评估基因突变对牦牛乳品质性状的影响，以期丰富牦牛重要经济性状的分子遗传研究基础。【方法】采用PCR-SSCP方法，检测甘南牦牛、天祝白牦牛、青海牦牛及野血牦牛DGAT1基因intron5-exon7和intron15-exon17区突变，分析基因突变对乳品质性状的影响。【结果】牦牛DGAT1基因intron5-exon7区发现c.562+32_c.562+33insC...
Effect of DGAT1, BTN1A1, OLR1, and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed
cattle genetic polymorphism fertility
2015/7/3
The impact of polymorphism of the diacylglycerol acyltransferase (DGAT1), butyrophilin (BTN1A1), oxidized low-density lipoprotein receptor (OLR1), and signal transducer and activator of transcription ...
Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental sires
cattle acyl-CoA diacylgycerol transferase1 K232A
2015/7/2
The aim was to evaluate the effect of polymorphisms in the promoter and in the coding region of the DGAT1 gene on the estimated breeding values (EBV) of Czech Simmental sires. The K232A polymorphism (...
Polymorphism identification in goat DGAT1 and STAT5A genes and association with milk production traits
milk yield fat percentage dairy goat SNP PCR-RFLP SSCP additive effect
2015/6/24
Polymorphisms of DGAT1 and STAT5A genes in Xinong Saanen and Guanzhong goat breeds were investigated. PCR-RFLP, SSCP, and DNA sequencing were used to identify three SNPs: DQ380250:g.407_408insC in the...
High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle
HRM analysis PCR-RFLP K232A polymorphism
2015/6/9
PCR-RFLP analysis is a common method for genotyping the DGAT1 K232A polymorphism in cattle. Our purpose was to develop a high resolution melting (HRM) assay in order to genotype the polymorphic allele...
The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires
bovine GH1 GHR DGAT1 milk production breeding value Holstein
2015/6/5
We analysed the relations of estimated breeding values (EBV) of 315 German Holstein sires to their genotypes in growth hormone gene (GH1), growth hormone receptor gene (GHR) and acylCoA-diacylglycerol...
甘南藏系绵羊DGAT1基因1617外显子多态性分析
藏系绵羊 DGAT1 PCRRFLP
2013/12/3
本研究旨在探讨甘南藏系绵羊(欧拉羊、甘加羊和乔科羊)及滩羊DGAT1基因1617外显子多态性，为开展藏系绵羊遗传分化、藏系绵羊与其他绵羊的亲缘关系、藏系绵羊DGAT1基因与肉质性状关联等方面的研究提供参考。采用PCRRFLP方法，检测501只绵羊DGAT1基因1617外显子SNP，并对主要遗传参数进行统计学分析。结果表明：①藏系绵羊DGAT1基因1617外显子均具有Alu Ⅰ酶切多态性，存...
以北京地区来自17个公牛家系的1 222头中国荷斯坦母牛为试验材料，采用PCR-RFLP技术对DGAT1基因进行了遗传多态性分析，共检测到KK、KA和AA 3种基因型，频率分别为0.143 2、0.609 7和0.247 1；采用混合动物模型对数据进行拟合，通过SAS（8.02）软件对5个产奶性状与DGAT1基因的关联程度进行了统计分析。结果表明，DGAT1不同基因型间的305 d产奶量、乳脂量、...
莱芜猪DGAT1基因的多态性研究
莱芜猪 二酰基甘油酰基转移酶1基因（DGAT1） 多态性
2008/4/9
二酰基甘油酰基转移酶1（Diacylglycerol acyltransferase,DGAT1）是催化酰基化的辅酶A和二酰基甘油合成甘油三脂的关键限速酶。莱芜猪具有较强的脂肪沉积能力，可能与DGAT1基因的活性有关。本研究对莱芜猪DGAT1的外显子6至外显子8共476bp的序列进行了克隆、测序（GenBank登录号：DQ289596）和多态性分析，分别在外显子6的第27bp位点和外显子8的第56...
莱芜猪DGAT1基因的多态性研究
莱芜猪 二酰基甘油酰基转移酶1基因（DGAT1） 多态性
2013/12/30
二酰基甘油酰基转移酶1（Diacylglycerol acyltransferase,DGAT1）是催化酰基化的辅酶A和二酰基甘油合成甘油三脂的关键限速酶。莱芜猪具有较强的脂肪沉积能力，可能与DGAT1基因的活性有关。本研究对莱芜猪DGAT1的外显子6至外显子8共476bp的序列进行了克隆、测序（GenBank登录号：DQ289596）和多态性分析，分别在外显子6的第27bp位点和外显子8的第56...