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Species Differentiation of Thermotolerant Campylobacters Based on Distinctive Banding Patterns Obtained by Multiplex PCR
Campylobacteriosis alimentary infection 23S rRNA
2015/3/4
The differentiation of thermotolerant Campylobacter spp. on the species level (C. jejuni, C. coli, C. lari, and C. upsaliensis) was provided. Identification is based on different banding patterns obta...
Real-time PCR with internal amplification control for the detection of Cronobacter spp. (Enterobacter sakazakii) in food samples
Cronobacter spp. (Enterobacter sakazakii) Real-time PCR Internal amplification control Detection Food
2012/8/14
Cronobacter spp. (Enterobacter sakazakii) is an opportunistic pathogen and is linked with life-threatening infections in neonates. The organism has been isolated from a wide variety of foods and envir...
Rapid detection of viable Listeria monocytogenes in chilled pork by real-time reverse-transcriptase PCR
Listeria monocytogenes Real-time reverse-transcriptase PCR Detection RNA Chilled pork
2012/8/14
The objective of this study was to develop an RNA-dependent real-time reverse-transcriptase PCR (realtime RT-PCR) method for the detection of Listeria monocytogenes in chilled pork without the need fo...
Inactivation of food spoilage bacteria by high pressure processing: Evaluation with conventional media and PCR–DGGE analysis
High pressure processing RNA extraction DGGE Active species Sliced cooked ham
2012/8/14
Microbial diversity and dynamic changes of sliced vacuum-packed cooked ham during refrigerated storage (0–90 days) after high pressure processing (400 MPa at 22 _C for 10 min) was investigated by usin...
Changes in the Composition of the Bacterial Flora on Tray-Packaged Pork during Chilled Storage Analyzed by PCR-DGGE and Real-Time PCR
bacterial community Pseudomonas PCR-DGGE the CT method tray-packaged pork
2012/8/13
In this study, a polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) was used to investigate the changes in the composition of the bacterial population of tray-packaged pork...
Identification of Wine Yeasts by PCR-RFLP without Previous Isolation on Plate
wine yeasts identification PCR-RFLP
2009/3/20
The population of wine yeasts during spontaneous must fermentation was characterized by direct 5.8S-ITS rDNA region amplification without previous plate isolation or enrichment. RFLP analysis was appl...
Mycobacterium avium subsp. paratuberculosis and M. a. avium detected by culture, IS900 and IS901 highly sensitive PCR in bulk tank milk from dairy herds in the Czech Republic between 2002 and 2004
Johne’s disease avian tuberculosis food safety zoonoses
2014/3/7
In this study, the possible presence was monitored of Mycobacterium avium subspecies paratuberculosis (MAP) and Mycobacterium avium subspecies avium (MAA) by means of culture examination and PCR in 25...
Cultivar Identification of Rice (Oryza sativa L.) by PCR Method and its Application to Processed Rice Products
rice DNA cultivar identification PCR processed rice product
2008/4/20
As the cultivars of rice affect markedly eating quality, processing suitability and price, identification or differentiation of rice cultivars is very important. The present authors developed suitable...
Optimisation of the PCR method for the detection of Campylobacter jejuni and Campylobacter coli in samples of ready-to-eat chicken meals
polymerase chain reaction internal control Campylobacter spp.
2014/3/3
This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken...
PCR-based detection of cow is milk in goat and sheep cheeses marketed in the Czech Republic
polymerase chain reaction cow is milk goat cheese sheep cheese
2014/3/17
A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow’s milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spi...