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Effect of thawing method on bull sperm survival in ejaculates frozen in 4 ml and 8 ml volumes
bovine semen large volumes semen processing sperm quality parameters
2018/11/23
The aim of this study was to evaluate the effect of different thawing protocols (slow (P1), medium (P2), and fast (P3)) on percentage of motile sperm (MOT) and percentage of sperm cells with intact me...
The Usefulness of Selected Physicochemical Indices, Cell Membrane Integrity and Sperm Chromatin Structure in Assessments of Boar Semen Sensitivity
Boar Chromatin Structure Sperm Membrane Integrity Sperm Morphology
2016/5/19
The present work describes experiments undertaken to evaluate the usefulness of selected physicochemical indices of semen, cell membrane integrity and sperm chromatin structure for the assessment of b...
Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction
Sex Determination Sexed Semen Multiplex Real-time polymerase chain reaction
2016/5/17
Gender selection is important in livestock industries; for example, female calves are required in the dairy industry. Sex-sorted semen is commonly used for the production of calves of the desired gend...
L-carnitine Supplemented Extender Improves Cryopreserved-thawed Cat Epididymal Sperm Motility
L-carnitine Epididymal Spermatozoa Cryopreservation Cat
2016/5/16
Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detr...
Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Sperm-mediated Gene Transfer Goat Sperm Cryopreservation Artificial Insemination (AI)
2016/5/3
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (...
Comparison of Viability, ATP and In vitro Fertilization of Boar Sperm Stored at 4??C in the Three Different Diluents
Fertilization Liquid Semen Oocyte Porcine
2016/4/28
This study was designed to develop a method of liquid storage of boar sperm at 4??C by using the modified Beltsville F5 (BF5) diluent with bovine serum albumin (BSA) and N-acetyl-D-glucosamine. Boar s...
Comparison of Genetic Parameter Estimates of Total Sperm Cells of Boars between Random Regression and Multiple Trait Animal Models
Genetic Correlation Boar Semen Random Regression Model Multiple Trait Animal Model
2016/4/28
The objective of this study was to compare random regression model and multiple trait animal model estimates of the (co) variance of total sperm cells over the active lifetime of AI boars. Data were p...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Maintenance of Sperm Characteristics and In vitro Developmental Rate of Embryos against Oxidative Stress through Antioxidants in Pig
Reactive Oxygen Species (ROS) Boar Semen Taurine Embryo Antioxidant Sperm Characteristic
2016/4/27
Oxidative stress is one of the major causes of failure of in vitro storage of boar semen. Reactive oxygen species (ROS) are one of the important mediators of oxidative stress during in vitro storage o...
Capacitation-associated Changes in Protein-tyrosine-phosphorylation, Hyperactivation and Acrosome Reaction in Guinea Pig Sperm
Acrosome Reaction Capacitation Hyperactivation Tyrosine Phosphorylation Semen
2016/4/27
The aim of this study was to evaluate the effects of Ca2+, HCO3- and BSA on the in vitro capacitation-associated protein tyrosine phosphorylation, hyperactivation and acrosome reaction in guinea pig s...
Effect of Monosaccharide L-fucose and Polysaccharide Fucoidan on Sperm L-fucosidase Activity and Relation to Sperm-oocyte Interaction in Pig
Pig Oocytes Chondroitin Sulfate Sperm Penetration In vitro
2016/4/25
Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fert...
Effects of Taurine on Sperm Characteristics during In vitro Storage of Boar Semen
Boar Semen Sperm Characteristic Hydrogen Peroxide (H2O2) Taurine Hypoosmotic Swelling Test (HOST)
2016/4/22
The objective of this study was to investigate the anti-oxidative effects of taurine on sperm characteristics for in vitro storage of boar semen. Semen was randomly divided into 10 groups in conical t...
The Role of Mercury in the Etiology of Sperm Dysfunction in Holstein Bulls
Mercury Bull Sperm Membrane and DNA Integrity Viability Acrosome Reaction
2016/4/20
A large number of toxicological substances and pharmacological and physical agents can cause reproductive intervention at the cellular and molecular level. The present study was designed to assess the...
Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes
In vitro Fertilization Pig Oocyte Liquid Boar Sperm
2016/4/18
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
Comparison of Semen Characteristics, Frozen-Thawed Sperm Viability, Testosterone Concentration and Embryo Development between Yorkshire Boar A and B
Sperm Viability Testosterone Embryo Yorkshire Boar
2016/4/15
This study was carried out to compare the semen characteristics, frozen-thawed sperm viability and testosterone concentration and in vitro fertilization (IVF) and development of in vitro matured pig o...