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本研究旨在比较牛A型多杀性巴氏杆菌高毒力株（PmCQ2）和低毒力株（PmCQ6）脂多糖（LPS）对小鼠巨噬细胞RAW264.7内TLR4信号通路的影响。选用体外培养RAW264.7细胞，经LPS刺激后检测细胞内TLR4信号激活及对TNF-α和IL-12p40表达的影响；并利用Western blot检测LPS对IκBα磷酸化及NF-κBp65活化的影响。
Effects of L-proline on the Growth Performance, and Blood Parameters in Weaned Lipopolysaccharide (LPS)-challenged Pigs
Growth Performance Blood Parameters Gastrointestinal Tract Digestibility Early Weaned Pigs
2016/5/17
This trail was conducted to study the effect of L-proline on the growth performance, and blood parameter in the weaned lipopolysaccharide (LPS)-challenged pigs. Thirty six pigs (9.13±0.85 kg) were ass...
Effects of Supplemental Glutamine on Growth Performance, Plasma Parameters and LPS-induced Immune Response of Weaned Barrows after Castration
Glutamine Weaned Piglets Growth Performance Castration
2016/5/10
Two experiments were conducted to investigate the effects of supplemental glutamine on growth performance, plasma parameters and LPS-induced immune response of weaned barrows after castration. In expe...
摘要 应用免疫荧光标记、基因转染等方法，观察脂多糖（LPS）刺激对单核细胞系Raw264.7细胞骨架的影响，探讨p38家族不同亚型对LPS诱导的细胞骨架蛋白微管蛋白与肌动蛋白变化的调控作用结果显示，未受LPS刺激的细胞富含微管蛋白，微管蛋白交联形成辐射状的交联丝网，丝网在细胞中分布均匀；LPS刺激后，微管蛋白募集在细胞膜、核膜周围；p38α、p38β、p38γ亚型的特异性抑制剂FHPI对LPS诱导...