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Systematical characterization of YUCCA gene family in five cotton species, and potential functions of YUCCA22 gene in drought resistance of cotton
棉花 YUC基因家族 生长素合成
2024/5/17
生长素在植物的正常生长发育中起着至关重要的作用。TAA/YUCCA基因家族编码色氨酸氨基转移酶（tryptophan aminotransferase），是生长素合成的关键酶。然而，棉花中YUCCA（YUC）基因家族尚未见报道。为了揭示YUC基因在棉花中的功能，我们系统地鉴定了五个棉种中的YUC基因家族，包括成员分析、特征描述、基因组内复制和共线关系。转录组学分析表明，GhYUCs参与了棉花的非生...
Identification and characterization of bovine mammary peptide transporters in response to tripeptide and lactogenic hormone treatment
bovine mammary gland PepT RT-PCR lactogen
2017/6/22
Oligopeptide transportation is mediated by the peptide transporter (PepT), which consists of two isoforms, PepT1 and PepT2. Because PepT play essential roles in amino acid metabolism and cell growth, ...
Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus)
Liaoning Cashmere Goat Keratin-associated Protein 11.1 (KAP11.1) Expression Pattern
2017/3/9
An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.
Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environments
Algerian cultivars growth habit low-temperature MAS VRN-H1 VRN-H2 FR-H2
2016/12/8
For the purpose of marker assisted selection (MAS), six Algerian cultivars and landraces developed locally or introduced from external breeding programs for semi-arid environments were marker characte...
Molecular characterization of snowdrop lectin (GNA) and its comparison with reported lectin sequences of Amaryllidaceae
bioinformatics biotic stress Galanthus nivalis gene cloning gene sequence insecticidal activity
2016/12/8
Plant lectins have become efficient sources of insect resistance in crops. The present study was conducted to identify, amplify, clone and characterize the plant lectin gene GNA. The lectin, present i...
Identification and characterization of the yls mutation in rice (Oryza sativa L.) with lower photosynthetic pigment content
chloroplast development map-based cloning SRP54 protein yellow-green leaf mutant
2016/12/8
Normal chloroplast development in rice is essential for photosynthesis and yield potential. To explore the physiological and molecular mechanism of chloroplast development, we isolated the rice mutant...
Characterization of the Methylation Status of Pax7 and Myogenic Regulator Factors in Cell Myogenic Differentiation
Pax7 Myogenic Regulatory Factors C2C12 DNA Methylation CpG Islands
2016/7/27
Epigenetic processes in the development of skeletal muscle have been appreciated for over a decade. DNA methylation is a major epigenetic modification important for regulating gene expression and supp...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...
1H NMR Characterization of Myoglobins Where Exogenous Ligands Replace the Proximal Histidine
Sperm whale myoglobin Site-directed mutagenesis Resonance assignment Heme Orientation Imidazole Cyanometmyoglobin Binding Mutants H-1-nmr
2016/5/23
The role of the proximal ligand in determining the structure and ligand binding properties of sperm whale myoglobin has been investigated using the mutant H93G(L), where the proximal histidine has bee...
Biochemical Characterization and Electron-Transfer Reactions of sym1,a Rhodobacter capsulatus Reaction Center Symmetry Mutant which Affects the Initial Electron Donor
Biochemical Characterization Electron-Transfer Reactions sym1 Rhodobacter capsulatus Reaction Center Initial Electron Donor
2016/5/23
A 51 bp section of the Rhodobacter capsulatus photosynthetic reaction center M subunit gene (nucleotides M562-M612 of the pufM structural sequence) encoding amino acids M187-M203 was replaced by the h...
Characterization of the Overall and Internal Dynamics of Short Oligonucleotides by Depolarized Dynamic Light Scattering and NMR Relaxation Measurements
Overall and Internal Dynamics Short Oligonucleotides Depolarized Dynamic Light Scattering NMR Relaxation
2016/5/23
The dynamics of three synthetic oligonucleotides d(CG)4, d(CG)6, and d(CGCGTTGTTCGCG) of different length and shape were studied in solution by depolarized dynamic light scattering (DDLS) and time-res...
Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry
Methanogen Anaerobic Digester 16S rRNA mcrA Methanobacterium formicicum
2016/5/20
A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, ...
Characterization and long-term trends of hypoxia in the northern Gulf of Mexico: Does the science support the action plan?
long-term trends of hypoxia the northern Gulf of Mexico
2016/5/18
We update and reevaluate the scientific information on the distribution, history, and causes of continental
shelf hypoxia that supports the 2001 Action Plan for Reducing, Mitigating, and Controlling ...
Characterization of greying, melanoma,and vitiligo quantitative inheritance in Old Kladruber horses
pigmentation skin neoplasm heritability genetic correlations spectrophotometry
2015/10/29
The paper deals with the greying process, melanoma, and vitiligo depigmentation occurrence in Old Kladruber horses (OKH). The study includes 376 animals of both sexes at the age of 1–25 years. The eva...