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SNP genotyping and population genomics from expressed sequences – current advances and future possibilities
SNP genotyping population genomics
2015/12/11
With the rapid increase in production of genetic data from new sequencing technologies,
a myriad of new ways to study genomic patterns in nonmodel organisms are currently
possible. Because genome as...
Discovery laboratories are dealing with DNA sequencer-based technologies which have seen great advancement over the past decade, resulting in several steps of the genotyping process becoming automated...
A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
Delineation Paralleled Genotyping
2015/4/1
Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important
food and feed grain crop legume, they have not yet been used for comprehensive molecula...
Rapid Genotyping of MSTN Gene Polymorphism Using High-resolution Melting for Association Study in Rabbits
MSTN HRM Direct Sequencing Rabbits Production Traits
2016/5/11
The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at −1...
Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays
Site Tiling Analysis accurate discovery
2015/12/11
High-throughput genotype data can be used to identify genes important for local adaptation in wild populations,
phenotypes in lab stocks, or disease-related traits in human medicine. Here we advance...
Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
Genotyping Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes PCR-RFLP Dried Blood DNA Extraction
2010/9/20
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
人类血小板抗原1～6系统同步基因分型的研究Study on the Simultaneous Genotyping of Human Platelet Antigens of 1, 2, 3, 4, 5, 6 System by PCR-SSP and Its Applications
序列特异性引物—PCR 人类血小板抗原 基因分型
2008/1/8
摘要
为研究采用PCR—SSP技术，建立可靠的人类血小板抗原HPA-1,2,3,4,5,6系统的同步基因分型方法，并以所建立的方法研究血小板抗原。设计合成18条序列特异性引物，探索最佳退火温度，通过调整引物浓度、Mg2＋离子浓度，使HPA-1~6系统等位基因在同一条件下进行同步扩增和扩增产物在同一凝胶中进行同步电泳。引物的特异性和灵敏度采用基因型已知的质控DNA进行验证。应用此方法，对2000年...
应用微卫星分型方法进行大熊猫父亲鉴定Paternity Assignment of Giant Panda by Microsatellite Genotyping
微卫星 基因分型 大熊猫 亲子鉴定
2007/12/31
摘要选用18对大熊猫特异的微卫星引物对成都动物园、成都大熊猫繁育研究基地的大熊猫幼子及其母亲和可疑父亲DNA作了PCR扩增。扩增产物在ABI 373测序仪上做基因扫描。 应用Genotyper 672 软件处理分型数据，经Cervus 2.0 软件对亲子关系进行分析。结果表明， 在母子关系确实的情况下，18个基因座联合的父亲鉴定排除概率E为0.999921，置信度为95%。而在母子关系不清的条件下...
Multilocus Genotyping to Study Population Structure in Three Buffalo Populations of India
Buffalo Microsatellites F-statistics Genetic Distance Correspondence Analysis
2016/4/21
Three buffalo populations viz. Bhadawari, Tarai and local buffaloes of Kerala were genotyped using 24 heterologous polymorphic microsatellite loci. A total of 140 alleles were observed with an average...
Three-step PCR and RFLP Genotyping of the Swine Ryanodine Receptor Gene Using Aged Single Hair Follicles Delivered by General Mail
Three-step (Booster and Nested) PCR Amplification RFLP Analysis Aged Hair Follicles RYR 1 Locus Genotyping
2016/4/11
We have developed a reliable and noninvasive method for swine genotyping of single locus nuclear gene with aged single hair follicles delivered by general mail. The method is based on booster and nest...