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为初步探究水曲柳根发育中PLT转录因子的基因功能及遗传调控机理,以水曲柳转录组数据为基础,参考拟南芥AtPLT2和AtPLT3基因序列,通过PCR技术从水曲柳中克隆了两个PLT转录因子基因,通过同源比对将它们命名为FmPLT2和FmPLT3,分别编码532和497个氨基酸残基。生物信息学分析表明,其相对分子质量分别为59和55 kDa,等电点分别为5.98和5.79,均为亲水性不稳定蛋白,均含有2...
通过对刚毛柽柳转录组分析,克隆获得了一条与S-腺苷甲硫氨酸合成酶(SAMS)基因同源性高的基因,命名为ThSAMS。序列分析结果表明:ThSASM基因全长cDNA为1 185 bp,编码394个氨基酸,编码蛋白相对分子质量为97.85 kDa,理论等电点为5.02。通过生物信息学分析表明,ThSASM基因编码的氨基酸与其他物种SAMS基因编码的氨基酸具有很高的同源性,其中与枣的同源性最高,达95%...
‘保佳俊’桃是河北农业大学选育的桃新品系。为加快其推广应用和进一步开展组织培养、基因工程、从分子水平探讨其抗褐化机理等研究,通过试验探讨不同培养基配比对‘保佳俊’茎尖培养的影响,筛选出适宜的培养基配方,建立其茎尖培养再生体系。结果表明,以‘保佳俊’茎尖为外植体,用75%的酒精消毒30s,再用0.1% HgCl2溶液消毒9min,成活率为50%,高于其他处理;继代培养阶段,MS基本培养基比1/2MS...
榆树种质资源的研究进展
榆树 种质资源 研究进展 展望
2018/7/4
榆树是我国北方的常见树种。结合国内外情况主要对榆树种质资源概况、繁殖技术、种质资源收集与育种、诱变育种与良种选育、应用价值及其展望等几个方面的研究进行综述,详细介绍了各个方面的近些年来的研究成果,并展望了榆树种质资源的研究、开发和利用前景,讨论了榆树种质资源研究、收集、保存和利用中存在的问题以及改进途径。
为了高效地营建云杉无性系种子园,以河北省木兰围场国有林场管理局龙头山国家重点落叶松良种基地的云杉为对象,通过野外调查的方法,对嫁接时间、嫁接人员技术熟练程度、嫁接方法、营建方式以及后期管理等关键技术进行研究。结果表明:在5月中旬至下旬嫁接成活率最高,为90.2%,比4月底至5月初嫁接提高46.4%,比6月初嫁接提高157.7%;经过严格训练的技术熟练人员的嫁接平均成活率为85.3%,未经培训的人员...
辽西地区油松人工纯林混交改造技术研究综述
油松混交 纯林改造 中幼龄林 研究综述
2018/7/4
为了促进辽西地区近成熟、成熟油松公益林混交改造技术发展,解决油松公益林现存的诸多问题,保障其未来的可持续发展,从混交改造的方式、树种、效果评价等3个方面对已有文献资料中油松人工纯林、中幼龄林期的混交改造技术相关研究内容进行了总结与探讨。在现有文献资料中,混交改造方式的研究缺少对油松带状或块状砍伐形状、面积大小的界定,未能依据具体的立地类型及微环境差异确定适宜的混交树种及详细的配置方法;混交改造树种...
为加快南方红豆杉珍贵用材遗传改良进程,基于速生且优质干材的珍贵用材选育目标,选择了6省9个优良种源点的46个优树,并在福建省明溪县开展优树子代测定试验,采用多目标决策方法进行优树家系与单株配合选择。结果表明,南方红豆杉种内存在丰富的遗传变异,子代间的树高、地径、冠幅、当年抽梢长度等生长性状及杈干率、高径比、侧枝数量、侧枝密度、最长侧枝长度和最大侧枝直径等形质性状差异显著;3年生幼树生长性状与形质性...
Weed suppression and early sugar beet development under different cover crop mulches
Beta vulgaris L. Chenopodium album L. conservation tillage cover crop mixture integrated weed management intercropping Stellaria media (L.)
2017/6/26
Field experiments were conducted at two locations in 2014–2015 and 2015–2016 to investigate the weed suppressive ability of cover crop mulches in sugar beets. Three cover crops and two cover crop mixt...
Biomass allocation, leaf gas exchange and nutrient uptake of hazelnut seedlings in response to Trichoderma harzianum and Glomus intraradices inoculation
Corylus avellana fungi growth rate leaf nutrients nursery stomatal conductance
2017/6/26
Mycorrhizal fungi form mutualistic symbioses with the roots of 80% of plants which increase growth and nutrient uptake for the host plants. This research was conducted to determine the effect of indiv...
Modelling individual tree diameter growth for Norway spruce in the Czech Republic using a generalized algebraic difference approach
base-age invariant approach Chapman-Richards function global parameters tree-specific parameters iterative nested regression National Forest Inventory
2017/6/26
Individual tree-based growth models precisely describe the growth of individual trees irrespective of stand complexity. These models are more useful than the stand-based growth models for effective ma...
Impact of logging intensity on stem density, basal area and biodiversity indices five years after logging in a Caspian hardwood forest
harvesting intensity forest biodiversity northern Iran
2017/6/26
The aim of the present study was to analyse the impacts of different logging intensities after five years from logging including non-logged, low (3.4 felled stems per hectare), medium (5.2 felled stem...
Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites
DNA analysis European beech populations gene reserves genetic distance
2017/4/6
Fagus sylvatica Linnaeus (European beech), the ecologically and economically most important broadleaved tree species in the Czech Republic, was strongly reduced in the past. Today there are efforts to...
元宝枫花中3种黄酮类成分的初步研究
高效液相色谱 元宝枫花 黄酮
2017/11/16
为了研究元宝枫花中药用成分及含量的问题,利用高效液相色谱技术,研究元宝枫不同单株花的槲皮素、山奈酚及异鼠李素3种黄酮类物质的含量。测试条件为SyncronisaQ-C18色谱柱,乙腈-0.2%磷酸(40:60)为流动相,流速0.9 mL/min,检测波长360 nm。结果显示槲皮素、山奈酚及异鼠李素的平均含量分别为0.696、0.229、1.207 mg/g,且株间差异明显。这意味着元宝枫花具有进...
Generalized height-diameter models for Fagus orientalis Lipsky in Hyrcanian forest,Iran
Oriental beech dominant height forest models evaluation validation
2016/10/14
In this study, 39 generalized height-diameter prediction models were developed for Oriental beech (Fagus orientalis Lipsky) in the Hyrcanian forest in Iran. Data were collected from 75 permanent sampl...