搜索结果: 16-30 共查到“知识库 食品科学技术基础学科其他学科”相关记录115条 . 查询时间(1.093 秒)
2011—2012年山东淄博市肉鸡屠宰场沙门菌污染情况分析
交叉污染 血清型 食源性致病菌
2015/11/24
调查肉鸡屠宰场加工环节中沙门菌的污染状况。方法 采集肉鸡屠宰场的入厂活鸡肛拭子样本,宰杀褪毛后整禽样本、预冷后整禽样本、预冷池水、分割刀具和案板、冷冻鸡肉样本以及工人手涂抹样本,按《食源性致病菌监测工作手册》方法检测沙门菌。结果 2011—2012年共检测样品896份,检出沙门菌293株,检出率为32.7%；鸡胴体的检出率高于鸡活体。2011年沙门菌优势血清型为印第安纳沙门菌、鼠伤寒沙门菌和肠炎沙...
人视网膜色素上皮细胞生长特性及可见光对其影响
hRPE细胞 生长特性 可见光损伤
2014/3/17
研究体外培养的人视网膜色素上皮细胞的生长特性及可见光对其影响.用倒置相差显微镜和透射电镜观察人视网膜色素上皮(hRPE)细胞形态和超微结构,用计数法和噻唑蓝(MTT)法绘制生长曲线.从光照时间、光照时有无血清、光照后是否换液、光照后培养时间这4方面考查可见光对hRPE细胞的损伤情况.实验结果表明,体外培养的hRPE细胞为多角形,有极性,细胞器正常,生长旺盛.(8423±359)lx的可见光光照2h...
基于电子舌技术对不同年份的化橘红的识别
电子舌 化橘红 年份 识别
2012/9/24
不同年份的化橘红功效和价格均存在差异,为了快速识别未知橘红样品的年份,采用电子舌对2001~2010 年的10个样品和一个盲样进行了分析.对原始数据采用主成分分析和判别因子分析.结果表明:主成分分析可以将不同年份的样品进行有效的区分,判别因子分析可以更好地区分不同年份的样品,并能进行盲样识别.在模式识别中,判别因子分析法的识别结果均优于主成分分析法,前者2 个判别因子的累计方差贡献量达到了 99....
60Co-γ射线辐照诱变筛选纳豆激酶高活耐热菌株
纳豆激酶 枯草芽孢杆菌 高活性 热稳定性
2013/9/3
为得到高活热稳定性的纳豆激酶生产菌,本研究采用60Co-γ射线辐照诱变纳豆激酶生产菌,以酪蛋白平板法作为活性筛选方法。经过辐照剂量筛选确认800 Gy为最适诱变剂量,在该剂量下正突变率最大,高达45%。经过800 Gy诱变共得到60株活性提高的菌株。不同温度热处理后,11株菌在65℃显示了很好的热稳定性。
吡哆醛激酶（pyridoxal kinase，PLK）是维生素B6关键代谢酶。前期研究克隆出家蚕Bombyx mori吡哆醛激酶cDNA，经序列比对发现几个重要且保守的氨基酸残基在此蛋白中被替换。为明确家蚕吡哆醛激酶分子若干特定位置上氨基酸残基在酶功能上的作用进行本研究。【方法】采用重叠延伸法对家蚕吡哆醛激酶Thr47，Asn121， Ile54， Arg88和Trp230氨基酸残基进行定点突变，...
Novel Bacteriocinogenic Lactobacillus plantarum Strains and Their Differentiation by Sequence Analysis of 16S rDNA, 16S-23S and 23S-5S Intergenic Spacer Regions and Randomly Amplified Polymorphic DNA Analysis
bacteriocin gene differentiation Lactobacillus plantarum ribosomal intergenic spacer region
2011/2/24
Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14
and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin
genes. A new combinatio...
Growth Inhibition of Film-Forming Yeasts During Production of Ume-Zuke (Pickled Japanese Apricots) by an Antimicrobial Fraction Extracted from Paprika Seeds
paprika seed film-forming yeast ume-zuke
2010/11/24
Paprika extract (50 to 100 μg/ml) obtained by suspending ground paprika seeds in water, adding ethanol (50%, v/v), and then filtering the suspension, inhibited the growth of film-forming yeasts (Kloec...
Application of Proteomics in Food Technology and Food Biotechnology: Process Development, Quality Control and Product Safety
proteomics food proteins and peptides food quality food safety
2010/12/21
Human food is a very complex biological mixture and food processing and safety are
very important and essential disciplines. Proteomics technology using different high-performance separation techniqu...
Effect of Organic Solvent on the Characteristics of Free and Immobilized Inulinase from Kluyveromyces marxianus ATCC 16045
organic solvent inulinase stability kinetic parameters
2010/10/14
The aim of this work is to evaluate the effects of the butyl acetate concentration on the
characteristics of free and immobilized inulinase from Kluyveromyces marxianus ATCC
16045. The mass fraction...
The Stability and Antioxidant Activity of Anthocyanins from Blueberry
highbush blueberry anthocyanins stability antioxidant activity
2010/8/11
Anthocyanins from highbush blueberry (Vaccinium corymbosum L.) have tremendous potential as natural colorants and functional food with pharmaceutical purposes in food applications. To exploit the pote...
微波处理切分莲藕防褐变技术的研究
微波 切分莲藕 PPO 防褐变
2010/2/22
为寻求一种简便、高效的微波处理切分莲藕的防褐变方法，经单因素和正交试验得出微波处理的最佳工艺参数为：微波火力为P50，微波处理时间为80s，藕片厚度为5-6mm。莲藕经此工艺加工后，抑制了藕片贮藏过程中PPO的活性，降低了藕片的褐变度，减少了淀粉、糖、蛋白质等主要营养成分损失，在4℃条件下可保存7d。
微波辅助提取玉米须黄酮工艺研究
玉米须 黄酮 微波辅助提取
2010/2/22
采用单因素分析结合正交试验的方法，研究了微波辅助提取玉米须黄酮的工艺条件，并与加热浸提法进行了比较。结果表明，微波辅助提取玉米须黄酮的最佳工艺条件为：以体积分数50%的乙醇为提取剂，料液比（g︰mL）1∶50，提取温度70℃，提取时间8 min。此条件下，黄酮提取率可达1.13 %。与加热浸提法相比，提取时间缩短约9/10，提取率提高约11 %。
Online Programs and Databases of Peptides and Proteolytic Enzymes – A Brief Update for 2007–2008
peptides peptidomics proteolysis bioinformatics computer databases world wide web
2010/3/24
Bioinformatics methods have become one of the most important tools in peptide science. The number of available peptide databases is growing rapidly. The number of online programs able to process pepti...
Potential Value of Hepatic Lipids from White Sea Bream（Diplodus sargus, L.）as a Good Source of Biomedical
Adriatic Sea Diplodus sargus fatty acid composition
2010/2/1
The aim of this study is to evaluate the potential value of hepatic lipids of the white sea bream, Diplodus sargus, as a source of important biomedical components. Fatty acid compositions of hepatic n...
苹果汁中溴氰菊酯残留的大孔树脂吸附分离（Separation of Deltamethrin Residue in Apple Juice with Macroporous Adsorption Resin）
溴氰菊酯残留 苹果汁 大孔吸附树脂
2009/8/11
为去除苹果汁中残留的溴氰菊酯,利用大孔吸附树脂对苹果汁中溴氰菊酯的吸附性能及参数进行了研究。通过树脂吸附溴氰菊酯的静态吸附试验, 对大孔吸附树脂进行了筛选,同时，在动态吸附试验的基础上，对最佳吸附动态条件进行了优化，结果表明：LS—803型树脂对溴氰菊酯的吸附能力最强, 其静态吸附时间为100min；通过正交试验确定了LS—803型树脂较佳的动态吸附条件为: 温度20℃、苹果汁质量浓度110g/L...