搜索结果: 1-14 共查到“multiplex PCR”相关记录14条 . 查询时间(0.125 秒)
Identification of Genes Conferring Resistance to Viral Diseases of Barley Using Multiplex PCR
BaYMV BaMMV BaYMV-2 BYDV Hordeum resistance genes
2016/4/5
This paper describes a rapid and cost-effective assay for identifying economically important barley resistance
genes based on multiplex PCR. A newly created reaction mixture is used for detecting th...
Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report
avian tuberculosis mycobacteriosis zoonosis
2015/4/8
We report three pigeons euthanized in a small household breeding facility, where there was suspicion of an avian tuberculosis outbreak. For rapid identification of Mycobacterium avium subsp. avium dir...
Species Differentiation of Thermotolerant Campylobacters Based on Distinctive Banding Patterns Obtained by Multiplex PCR
Campylobacteriosis alimentary infection 23S rRNA
2015/3/4
The differentiation of thermotolerant Campylobacter spp. on the species level (C. jejuni, C. coli, C. lari, and C. upsaliensis) was provided. Identification is based on different banding patterns obta...
Development of the Multiplex PCR Detection Kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7
foodborne pathogens rapid detection rapid screening
2011/2/24
This review describes the development of the multiplex PCR detection kit for Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in food samples. To develop a detection assay, our re...
Multiplex PCR assay to detect rust resistance genes Lr26 and Lr37 in wheat
leaf rust Lr26 Lr37 multiplex PCR wheat
2014/3/10
Multiplex PCR was developed and optimized for simultaneous detection of wheat leaf rust resistance genes Lr26 and Lr37. The presence of the genes was analyzed in 21 winter wheat cultivars registered i...
Rapid and simultaneous detection of 6 types of human herpes virus (herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus, human herpes virus 6A/B, and human herpes virus 7) by multiplex PCR assay
human herpes virus herpes simplex virus
2009/12/16
A multiplex PCR assay was developed that enabled the simultaneous detection of DNA from 6 types of human herpes virus, HSV-1/2, VZV, EBV, CMV, HHV-6A/B, and HHV-7, using appropriate primer sets and co...
Identification of New Cry Genes in Bacillus thuringiensis Isolates by Multiplex PCR and RFLP
Bacillus thuringiensis gene characterization biological control multiplex PCR Cry toxins
2010/11/12
Proteins produced by the bacteria Bacillus thuringiensis are widely used for pest control in
agriculture and reforestation. Those proteins are produced by genes known as cry genes. Some members of th...
Analysis of Cell-Free Fetal DNA from Maternal Plasma and Serum Using a Conventional Multiplex PCR:Factors Influencing Success
Noninvasive prenatal diagnosis maternal plasma maternal serum fetal gender determination conventional multiplex PCR
2009/6/23
Recent technology enables the use of cell-free fetal DNA in maternal plasma and serum for noninvasive prenatal genetic diagnosis. This study was designed to evaluate factors most likely to influence t...
银染法检测STR复合扩增产物应用于法医个体识别的初步研究A Study of Forensic Individual Identification Used STR Locus With Silver Staining and Multiplex PCR Methods
短串联重复序列(STR) 复合扩增 个体识别
2008/1/29
摘要为提高法医生物检材利用率和检案效率，研制银染法能检测的4个基因座和6个基因座STR复合扩增试剂。对一例轮奸案检材DNA，运用同步复合PCR技术对CSF1PO、TPOX、THO1和vWA(A组)4个STR基因座；D18S51、D7S820、D13S317、D5S818、D3S1358 5个STR基因座和Amelogenin(B组)性别基因座分别进行4个基因座和6个基因座的PCR复合扩增，两组扩增...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC)；结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...
Rapid Detection of Different Serovares of Salmonella entrica by Multiplex PCR
PCR Salmonella enterica Typhoid fever
2010/4/12
Background: Typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. Aim of current study was to evaluate a shortened time –Multiplex...
Detection of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum by Multiplex PCR in Semen Sample of Infertile Men
Infertility Male Chlamydia trachomatis Mycoplasma hominis Ureaplasma urealyticum
2010/4/9
Background: The aim of this study was to detect Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyti¬cum from semen samples of infertile men by Multiplex PCR and investigation of infl...
Detection of Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum by Multiplex PCR in Semen Sample of Infertile Men
Infertility Male Chlamydia trachomatis Mycoplasma hominis Ureaplasma urealyticum
2010/4/9
Background: The aim of this study was to detect Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyti¬cum from semen samples of infertile men by Multiplex PCR and investigation of infl...