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Transcriptome profile of one-month-old lambs’ granulosa cells after superstimulation
Lamb Granulosa Cells Sheep RNA Seq Superstimulation
2017/3/7
Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte m...
Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro
granulosa cells aromatase inhibitor proliferation
2015/6/8
The objective of the present work was to study the effect of a nonsteroidal aromatase inhibitor (fadrozole) on proliferation, estradiol production, aromatase expression and telomerase activity (TA) in...
Serum deprivation affects secretory activity of cultured porcine ovarian follicles and granulosa cells and their response to hormones
stress malnutrition IGF-I
2015/4/1
The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum depriv...
Effect of polychlorinated biphenyls on the secretion of oxytocin from luteal and granulosa cells in cow: possible involvement of glucocorticoid receptors
polychlorinated biphenyls oxytocin glucocorticoid receptors ovary cow
2015/3/5
Polychlorinated biphenyls (PCBs) stimulate oxytocin secretion from bovine granulosa and luteal cells. Since oxytocin on the one hand is released from ovarian cells by cortisol and on the other hand PC...
Validation of Gene Silencing Using RNA Interference in Buffalo Granulosa Cells
RNAi Transfection CYP19 Gene 17beta- Estradiol Buffalo Transcript Abundance
2016/5/9
Silencing of a specific gene using RNAi (RNA interference) is a valuable tool for functional analysis of a target gene. However, information on RNAi for analysis of gene function in farm animals is re...
Influence of Nitric Oxide on Steroid Synthesis, Growth and Apoptosis of Buffalo (Bubalus bubalis) Granulosa Cells In vitro
Apoptosis Buffalo Granulosa Cell Nitric Oxide Steroidogenesis
2016/5/6
Objective of this study was to examine the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor on steroid synthesis, growth and apoptosis of buffalo granulosa cells (GCs) in vitro. Follicu...
Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells
c-FLIP(L) pAcGFP-N l Recombinant Plasmid Follicular Granulosa Cell
2016/5/4
FLICE inhibitory protein (FLIP) is one of the important anti-apoptotic proteins in the Fas/FasL apoptotic path which has death effect domains, mimicking the pro-domain of procaspase-8. To reveal the i...
Effects of Daidzein on mRNA Expression of Bone Morphogenetic Protein Receptor Type I and II Genes in the Ovine Granulosa Cells
Daidzein Granulosa Cells Cell Proliferation BMP Receptor Genes
2016/5/4
Daidzein, a natural isoflavonoid phytoestrogen, structurally resembles estradiol (E2) and possesses estrogenic activity. This study was designed to test the hypothesis that daidzein may mimic the effe...
Culture of Chicken Granulosa Cells from Small Yellow Follicles:A Suitable Culture System
chicken granulosa cells culture system
2009/7/31
In the chicken ovary, one small yellow follicle (SYF) is selected daily to enter the hierarchy and ovulates a few days later. Cellular changes that occur in these follicles may provide an understandin...
Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)
Coturnix japonica follicle FSH granulosa cells ovary perivitelline membrane quail ZPC
2009/7/31
Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of two glycoproteins. Previous studies have indicated that one of the components, a glyco...
Effect of Arachidonic Acid on Production of Laminin and Connexin of Granulosa Cells from Chicken Pre-hierarchical Follicles
Granulosa Cell Arachidonic Acid Laminin Connexin 43 Chicken
2016/4/29
Arachidonic acid (AA) is a polyunsaturated fatty acid that is a normal constituent of membrane lipids in animal cells. In addition to its role as a precursor of prostaglandins, AA itself may play an i...
Changes in Number of Granulosa Cells, Follicular Fluid Levels and Diameter of Oocytes during Folliculogenesis in Pre-pubertal Gilts at Marketing Weight
Porcine Follicle Size Follicular Fluid Oocyte Granulosa Cell
2016/4/18
The follicles (1.8 to 7.8 mm in diameter) were recovered from the ovaries in marketed pigs and the number of granulosa cells, the diameter of oocytes obtained from different development stages of the ...
Effect of Follicular Fluid Proteins and Gonadotropins on Progesterone Secretion by Buffalo Granulosa Cells In vitro
Buffalo Ovary Follicular Fluid Proteins Granulosa Cells Progesterone Secretion
2016/4/18
In the mammalian ovary the follicular fluid contains proteins and peptides which play an important role in growth, development and maturation of oocytes. The gonadotropins and some other factors work ...
Albumin Fractions from Different Species Stimulate In Vitro Progesterone Production by Granulosa Cells in Buffalo
Serum Albumin Granulosa Cells In Vitro Steroidogenesis Buffalo
2016/4/12
The ovarian follicular fluid was found to contain steroidogenesis stimulatory protein similar to albumin from human and buffalo. Therefore, the albumins from various species, commercial and purified, ...
Intracellular Mechanisms of Growth Hormone Action on Apoptosis in Cultured Porcine Ovarian Granulosa Cells
Growth Hormone Protein Kinase G Tyrosine Kinase CDC Kinase Apoptosis Ovary
2016/4/11
The aims of this study were to detect spontaneously occurring apoptosis in cultured porcine ovarian cells, to examine the role of growth hormone (GH), tyrosine kinase (TK), protein kinase G (PKG) and ...