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中国科学院深圳先进技术研究院专利：具有FRET效应的还原敏感型荧光纳米胶束及其制备方法
2017年6月19日，英国皇家化学会主办刊物Chemical Communications（ChemComm）以封面论文（Inside Front Cover）的形式在线发表了中国科学院生物物理研究所柯莎（Sarah Perrett）研究组题为A Co-expression Strategy to Achieve Labeling of Individual Subunits within a D...
Scientists optimize FRET-FLIM to study activity of two signaling molecules within a single dendritic spine
Scientists FRET-FLIM two signaling molecules single dendritic spine
2016/11/4
An ongoing challenge for scientists working to understand the brain is being able to see all its parts. Researchers have spent centuries developing better imaging techniques to see beyond the abilitie...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
蛋白质传感器作为一种分析蛋白质与其他分子之间作用力的有效手段已经越来越显示出其优点, 但是蛋白质在基底表面的变性问题对于研究者来说仍然是一个挑战. 作者介绍了一种具有高灵敏度的蛋白质传感器, 即利用DNA引导固定(DDI)方法, 将DNA- 蛋白质复合物通过DNA双链特异性杂交固定在硅基底表面, 从而有效保持了蛋白质的活性, 并且引入荧光共振能量传递(FRET)技术以及电势扫描发卡去杂交(SPHD...
A New FRET-Based Sensitive DNA Sensor for Medical Diagnostics using PNA Probe and Water-Soluble Blue Light Emitting Polymer
FRET-Based Sensitive DNA Sensor Medical Diagnostics PNA Probe Water-Soluble Blue Light Emitting Polymer
2009/9/7
A reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Pol...
SHOULD WE FRET ABOUT OUR Low NET NATIONAL SAVING RATE?
NET NATIONAL SAVING RATE World War II GNP
2008/12/2
Relative to most other industrial countries, the United States has
recorded low ratios of saving and investment to GNP since the end
of World War II, The official accounts show a further decline in ...
cAMP依赖的蛋白激酶（ protein kinase A, PKA）在细胞生长与分化过程中扮演重要角色，特别是在调节Ras信号通路引起的细胞增殖效应中起着重要作用。为了在活细胞内动态观察表皮生长因子（epidermal growth factor, EGF）对PKA的作用，采用一种可以检测PKA酶活性的报告蛋白（A-kinase activity reporter, AKAR）———这种报告蛋白...
利用FRET技术在活细胞内研究红景天甙对Aß25-35诱导PC12细胞凋亡的抑制作用
红景天甙(Salidroside) PC12细胞(PC12 cells) 细胞凋亡(apoptosis) Aß 25－35 荧光共振能量转移(FRET)(Fluorescence Resonance Energy Transfer (FRET))
2008/5/19
为研究红景天甙(Salidroside)对ß淀粉样肽25-35（ß amyloid peptide25-35, Aß25-35）诱导PC12细胞凋亡的抑制作用，本文采用Cell Counting Kit-8 (CCK-8)分析细胞的存活率，通过光镜检测细胞形态以及Hoechst染色检测细胞核固缩，利用荧光共振能量转移(Fluorescence Resonance...
Rho small GTPase regulates the stability of individual focal adhesions: a FRET-based visualization of GDP/GTP exchange on small GTPases
cell motility cell-substratum adhesion Rac
2010/12/2
RhoA and Rac1 are small GTPases primarily involved in cytoskeletal remodeling. Many biochemical studies have suggested that they are also key organizers of cell-substrate adhesion. Recently, fluoresce...
用FRET技术研究TLR4和MD-2的相互作用
2007/7/25
Toll样受体4(TLR4)作为一种启动天然免疫反应的跨膜受体，目前已明确其在机体对脂多糖 (LPS)介导的信号传导中发挥关键作用[1][2][3][4]。然而，进一步的研究发现TLR4的功能需要有髓样细胞分化蛋白2(MD-2)的辅助。在只有TLR4而没有MD2存在的情况下，细胞对LPS不发生反应或反应微弱。但至今还没有直接的证据证实活体细胞膜上的TLR4与MD-2是否相互作用并形成复合物共同参与...
信号肽-FRET荧光蛋白载体的构建及在NIH3T3细胞中的表达
2007/7/25
荧光共振能量转移(fluorescence resonance energy transfer，FRET)是一项应用能量转移来测量分子内和分子间的距离的技术。当供体发射的荧光与受体发色团分子的吸收光谱重叠，并且两个探针在几个原子直径范围以内时，就会发生一种非放射性的能量转移，这种现象就叫FRET[1]。绿色荧光蛋白(green fluore- scence protein，GFP)发现后，它的许多...
Effect of Electrostatic Interactions on Binding and Retention of DNA Oligomers to PNA Liposomes Assessed by FRET Measurements
Peptide nucleic acid(PNA) Liposome DNA adsorption Biosensors FRET
2009/12/22
The FRET signal was maintained in the presence of 10-,20-,40-,
and 60-mer DNA inhighionic-strength buffer,showing that the driving force for the desorption is electrostatic repulsion between the boun...