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Human and Ram Seminal Plasma Both Contain a Calcium-Dependent Regulator Protein
Spermatozoa initiation of motility Ca2+-dependent regulator Ca2+-ATPase epididymis
2009/2/19
Biochemical aspects of post-testicular sperm maturation have been studied. Partial purification of either human or ram seminal plasma yields a heat-stable, acidic protein which is a Ca2+-dependent...
Evidence for a role of intracellular-calcium release in nitric oxide-stimulated relaxation of the rabbit corpus cavernosum
Calcium erection thapsigargin ryanodine
2009/2/17
Erection is mediated by relaxation of the smooth muscle elements within the sinusoids of the corpus cavernosum. Although cavernosal relaxation can be mediated by a variety of mechanisms including pu...
Voltage-dependent calcium channels and Gi regulatory protein mediate the human sperm acrosomal exocytosis induced by N-acetylglucosaminyl/mannosyl neoglycoproteins
Carbohydrate G-protein acrosome reaction VDCC neoglycoproteins
2009/2/16
Mammalian spermatozoa must undergo an exocytotic event during fertilization, the acrosome reaction (AR). In most species studied this process is induced by specific glycoproteins of the oocyte ext...
Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge
Milk egg yolk-Tris indo-1 acetoxymethyl ester cold
2009/2/13
Bovine spermatozoa are commercially cryopreserved by diluting the cells in media, known as extenders, followed by slow cooling and freezing. Previous work has shown that this process of cryopreser...
Intracellular calcium accumulation and responsiveness to progesterone in capacitating human spermatozoa
Spermatozoa [Ca2+]i progesterone capacitation
2009/2/7
Progesterone induced a rapid, long-lasting, dose-dependent increase of intracellular free calcium concentration ([Ca2+]i) in human sperm capacitated overnight. This effect was not counteracted by th...
Extracellular calcium and luteinizing hormone effects on 22-hydroxycholesterol used for testosterone production in mouse Leydig cells
Extracellular calcium luteinizing hormone testosterone production mouse Leydig cells
2009/2/7
The effect of extracellular calcium on testosterone synthesis in response to luteinizing hormone (LH) or 22-hydroxycholesterol (22-OH-C) by isolated adult mouse Leydig cells was studied. Leydig ce...
Sperm calcium levels and chlortetracycline fluorescence patterns are related to the in vivo fertility of cryopreserved bovine semen
Sperm calcium levels chlortetracycline vivo fertility cryopreserved bovine semen
2008/12/25
Cryopreserved bovine semen is less fertile than fresh semen for reasons that have not been fully elucidated. Cryopreservation is known to disrupt the sperm plasma membrane and it induces premature c...
Progesterone-induced calcium influx in cynomolgus monkey（Macaca fascicularis）spermatozoa
Progesterone-induced calcium cynomolgus monkey spermatozoa
2008/12/25
For in vitro capacitation to occur in cynomolgus monkey (Macaca fascicularis) spermatozoa, there is an absolute requirement for exogenous stimulation with the sperm activators, caffeine (1 mM) and d...
Measurement of intracellular calcium concentration and plasma membrane potential in human spermatozoa using flow cytometry
Measurement intracellular calcium concentration plasma membrane potential human spermatozoa
2008/12/25
We report 2 novel approaches using flow cytometry to measure intracellular calcium concentration and plasma membrane potential in human spermatozoa. Both approaches have the potential to measure dif...
Calcium-Modulated Rod Outer Segment Membrane Guanylate Cyclase Type 1 Transduction Machinery in the Testes
ROS-GC1 GCAP1 S100B signal transduction
2008/12/24
The importance of the second messengers, Ca2+ and cyclic GMP, for the process of fertilization is well established; the mechanisms for their intracellular regulations in the testes are, however, poo...
Successful Pregnancy and Delivery Following Intracytoplasmic Injection of Frozen-Thawed Nonviable Testicular Sperm and Oocyte Activation With Calcium Ionophore
Successful Pregnancy Delivery Intracytoplasmic Injection Frozen-Thawed Nonviable Testicular Sperm Oocyte Activation
2008/12/24
Several pregnancies have been reported using frozenthawed testicular sperm (Fisher et al, 1996; Podsiadly et al, 1996; Khalifeh et al, 1997), stressing the role of sperm motility for successful outc...
Androgens Induce Increases in Intracellular Calcium Via a G Protein–Coupled Receptor in LNCaP Prostate Cancer Cells
5-Dihydrostestosterone Ca2+ GPCR
2008/12/24
The receptor mechanism of testosterone-induced nongenomic Ca2+ signaling in prostate cancer cells is poorly understood. In this study we investigated androgen-induced intracellular Ca2+ increa...
The Effect of Calcium Channel Blockers on Male Reproductive Potential
Calcium Channel Blockers Male Reproductive Potential
2008/12/23
The potential impact of calcium channel blockers on male fertility has been a topic of discussion for some time. In this edition of Androlog, Dr Grace Centola describes her efforts in the management o...
A Cyclic Adenosine 3',5'-Monophosphate Stimulates Phospholipase C1-Calcium Signaling via the Activation of Tyrosine Kinase in Boar Spermatozoa
Sperm cAMP SYK PLC calcium store
2008/12/23
The aim of this study was to reveal a downstream part of the intracellular signaling that is mediated by cyclic adenosine monophosphate (cAMP)-dependent tyrosine kinases, including spleen tyrosine (...
Lack of Voltage-Dependent Calcium Channel Opening During the Calcium Influx Induced by Progesterone in Human Sperm. Effect of Calcium Channel Deactivation and Inactivation
Intracellular calcium mammalian sperm membrane potential membrane potential-sensitive dyes
2008/12/22
Progesterone induces calcium influx and acrosomal exocytosis in human sperm. Pharmacologic evidence suggests that voltage-dependent calcium channels (VDCCs) are involved. In this study, membrane p...