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The sperm chromatin structure assay. Relationship with alternate tests of semen quality and heterospermic performance of bulls
sperm chromatin structure assay alternate tests semen quality heterospermic performance
2009/2/23
Data obtained by the sperm chromatin structure assay (SCSA) on spermatozoa from nine bulls were correlated with fertility, measured by heterospermic performance (-0.94, P less than 0.01) and by al...
Effects of heat stress on mouse testicular cells and sperm chromatin structure
Testicular heat stress SCSA
2009/2/17
Scrotal regions of mice were exposed to a 38.0, 40.0, or 42.0 degrees C (+/-0.1) H2O bath for 60 minutes to determine the effects of elevated temperatures on testicular cells and sperm chromatin str...
Studies on sperm chromatin structure alterations and cytogenetic damage of mouse sperm following in vitro incubation. Studies on in vitro-incubated mouse sperm
sperm chromatin structure alterations cytogenetic damage of mouse sperm
2009/2/12
Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metapha...
Comparative study of cytochemical tests for sperm chromatin integrity
Comparative study cytochemical tests sperm chromatin integrity
2008/12/26
Tests were carried out on sperm from 40 fertile and infertile men to evaluate 2 DNA in situ denaturation methods using acridine orange (AO; the modified Rigler-Roschlau method and the Tejada method), ...
Characteristics of human sperm chromatin structure following an episode of influenza and high fever：a case study
Characteristics human sperm chromatin structure episode influenza high fever
2008/12/25
Semen samples from a fertile patient presenting with influenza and a 1-day fever of 39.9 degrees C were obtained and analyzed at 18-66 days postfever (dpf) for sperm nuclear proteins, DNA stainabi...
The Relationship Between Sperm Morphology and Chromatin Integrity in the Koala（Phascolarctos cinereus）as Assessed by the Sperm Chromatin Dispersion Test（SCDt）
DNA damage marsupial
2008/12/24
Koala (Phascolarctos cinereus) sperm nuclei show a tendency to swell after cryopreservation, but it is uncertain whether this phenomenon is associated with DNA fragmentation. In this study, we va...
Effects of the Chemotherapy Cocktail Used to Treat Testicular Cancer on Sperm Chromatin Integrity
Sperm DNA DNA strand breaks
2008/12/24
The incidence of testicular cancer has increased dramatically over the past 50 years. Advances in treatment, which include the coadministration of bleomycin, etoposide, and cis-platinum (BEP),...
Increased Aneuploidy Rate in Sperm With Fragmented DNA as Determined by the Sperm Chromatin Dispersion（SCD）Test and FISH Analysis
Human fragmentation
2008/12/24
Previous studies suggest that sperm DNA fragmentation may be associated with aneuploidy. However, currently available tests have not made it possible to simultaneously perform DNA fragmentatio...
Seasonal Changes in Sperm Chromatin Condensation in Ram（Ovis aries）, Iberian Red Deer （Cervus elaphus hispanicus）, and Brown Bear（Ursus arctos）
SCSA season
2008/12/24
The effect of seasonality (temperate environment, Spain) on the chromatin status of ovine (Churra breed), Iberian red deer, and brown bear spermatozoa was studied. This work aims to improve genet...
Infertile Men With Varicocele Show a High Relative Proportion of Sperm Cells With Intense Nuclear Damage Level, Evidenced by the Sperm Chromatin Dispersion Test
Human sperm DNA fragmentation
2008/12/23
The frequency of sperm cells with fragmented DNA was studied in a group of 18 infertile patients with varicocele and compared with those obtained in a group of 51 normozoospermic patients, 103 patie...
Effect of Antioxidant Intake on Sperm Chromatin Stability in Healthy Nonsmoking Men
Fertility spermatozoa nutrition oxidative stress DNA fragmentation
2008/12/23
Oxidative stress is detrimental to sperm function and a significant factor in the etiology of male infertility. This report examines the association between dietary and supplementary intake of the...
Variability and Laboratory Factors Affecting the Sperm Chromatin Structure Assay in Human Semen
Flow cytometry DNA integrity spermatozoa acridine orange
2008/12/23
During the past decade, the sperm chromatin structure assay (SCSA) has become an important tool for assessing semen quality in the human andrology laboratory. The SCSA uses the metachromatic propert...
Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
Artificial insemination semen quality motility chlortetracycline viability SCSA
2008/12/22
We hypothesized that cryopreservation and incubation in conditions that mimic the female genital tract following insemination increases the susceptibility of ram sperm DNA to denaturation. Ram spe...
The Sperm Chromatin Dispersion Test: A Simple Method for the Determination of Sperm DNA Fragmentation
Structure human sperm decondensation
2008/12/18
Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation...