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scTRAIL在E.coli中的重组表达及抗肿瘤活性的研究
肿瘤坏死因子相关凋亡诱导配体 单链化 大肠埃希菌 重组表达 抗肿瘤活性
2020/8/10
重组表达人单链TRAIL（single-chain TRAIL，scTRAIL）及研究其在抗肿瘤中的作用。方法：通过重组PCR获得编码scTRAIL的基因序列，构建含编码scTRAIL基因的重组质粒，将重组表达质粒转化到大肠埃希菌(E.coli)BL21（DE3），通过IPTG诱导，破碎细菌，上清行Amylose Resin亲和层析初步纯化融合蛋白。通过MTT法检测融合蛋白对人胰腺胆管上皮细胞SW...
High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
Fibroblast Growth Factor 2 Codon Protein E. coli
2016/1/26
Background: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration.
Objectives: The ob...
High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
Integrons Urinary Tract Infections Multidrug Resistant Escherichia coli
2016/1/26
Background: Most urinary tract infections (UTI) are caused by Escherichia coli. Integrons have an important role in distributing antibiotic resistance genes among bacteria.
Objectives: The aim of thi...
Penn Researchers Discover Why E.coli Move Faster in Syrup-like Fluids Than in Water
Penn Researchers E.coli Syrup
2015/12/7
Swimming in a pool of syrup would be difficult for most people, but for bacteria like E. coli, it’s easier than swimming in water. Scientists have known for decades that these cells move faster a...
Shiga Toxigenic Escherichia coli in Iranian Pediatric Patients With and Without Diarrhea: O-Serogroups, Virulence Factors and Antimicrobial Resistance Properties
Microbial Sensitivity Tests Virulence Factors Iran Shiga Toxigenic Escherichia coli
2015/11/9
Background: Shiga-toxigenic Escherichia coli is an important human pathogen cause of diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura in humans is a sig...
Virulence Factors and O-Serogroups Profiles of Uropathogenic Escherichia Coli Isolated from Iranian Pediatric Patients
Uropathogenic Escherichia Coli Pediatrics Iran
2015/10/10
Background:: Uropathogenic Escherichia coli O- Serogroups with their virulence factors are the most prevalent causes of UTIs.
Effect of pyk gene location on constructing dATP conversion E. coli strain
pyk Gene Location dATP Conversion Strain 5-Untranslation Regions
2013/1/28
A dATP conversion E. coli strain could be constructed when both pyk and adk1 gene were expressed successfully. pyk gene encodes pyruvate kinase (PK) could be expressed, when inserted it before adk1 ge...
Detection of Stx2 Gene of Escherichia coli and Elevated Levels of Fecal Bacteria in the Cattle Farming Regions of Lake Oconee
Fecal Bacteria Oconee Watershed qPCR Eschericha coli Tuf Gene Bacteroidale
2013/1/29
The presence of Total coliform, Eschericha coli and enterococci were enumerated in the cattle farming areas of the Oconee Watershed using colilertTM and enterolertTM IDEXX plates, respectively. Microb...
Experimental Intramammary Infection with a Strain of Escherichia coli Isolated from a Cow with Persistent E. coli Mastitis
Escherichia coli Intramammary Experimental Infection Persistent Mastitis Dairy Cows
2013/2/19
Transient E. coli intramammary infections (IMI) are usually associated with rapid onset of clinical signs including mammary gland swelling and abnormal milk with rapid clearance of bacteria from milk....
Rapid Detection of Escherichia coli O157: H7 by Fluorescent Amplification–Based Specific Hybridization (FLASH) PCR
E.coli O157:H7 Stx-1 gene Detection FLASH-PCR
2015/9/28
Background: Escherichia coli O157:H7 is an enteric pathogen which can be frequently found asymptomatically in ruminant mammals, but can cause diseases from mild diarrhea to hemolytic uremic syndrome i...
Genotypic Analysis of E. coli Strains Isolated from Patients with Cystitis and Pyelonephritis
Uropathogenic Escherichia coli Pyelonephritis Cystitis Electrophoresis Genetic patterns
2015/9/25
Background: Urinary tract infection is the most common health problem affecting millions of people each year, mainly caused by a large genetically heterogeneous group of Escherichia coli called uropat...
Germany Outbreak of Escherichia Coli, Lessons to Be Learned
Germany Outbreak Escherichia Coli Lessons Be Learned
2015/9/23
One of the most severe outbreaks of Escherichia coli has recently occurred in Europe.1 The epidemic was started in Germany but now has affected more than 16 countries with more than 3200 reported dise...
Anti-Microbial Resistance Profiles Of E. Coli Isolated From Free Range Chickens In Urban And Rural Environments Of Imo State,Nigeria
Anti-microbial resistance E.coli free-range chicken antibiotics Nigeria
2015/8/1
Information on the resistance profiles of normal intestinal flora of extensively reared chickens that hardly receive antibiotics in the developing countries can serve as important means of understandi...
Association of Virulent Genes hly, sfa, cnf-1 and pap with Antibiotic Sensitivity in Escherichia coli Strains Isolated from Children with Community-Acquired UTI
E coli UTI pap hly sfa cnf-1 Drug resistance
2015/9/22
Background: Recent studies have suggested that the decrease in the pathogenicity of E. coli is due to acquisition of resistance to some antibiotics. This study was performed to investigate four virule...
THE FIRST REPORT ON THE ISOLATION OF ENTEROTOXIGENIC ESCHERICHIA COLI AS A CAUSE OF INFANTILE DIARRHEA IN IRAN
Enterotoxigenic Infantile diarrhea Enteropathogenic E.coli
2009/12/28
The role of enterotoxigenic E.coli as a causative agent in diarrheal disease was studied among 100 cases of infant and children 0-2 years of age. Routine bacteriological methods were used for identifi...