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Genealogical analysis of the Gochu Asturcelta pig breed:insights for conservation
genetic variability pedigree analysis founder contribution conservation of genetic stock
2016/3/24
The Breeders Association of Gochu Asturcelta (ACGA) initiated a recovery programme with six founders (three boars and three sows) in 2002. Information provided by a total of 3156 records (515 litters,...
llele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds
quantitative trait locus GHRL pigs polymorphism genetic marker
2015/10/9
The allelic frequency of c.335A>C polymorphisms in the porcine ghrelin/obestatin prepropeptide (GHRL) gene was surveyed among six pig breeds and two subspecies of wild boars. The c.335C was the most f...
Genetic variation at RYR1, IGF2, FUT1, MUC13, and KPL2 mutations affecting production traits in Chinese commercial pig breeds
China commercial pig breed genetic variation economically important markers
2015/6/24
The identification of causative mutations affecting economically important traits has benefited the worldwide pig industry. We investigated the genetic variation at five loci including RYR1, IGF2, FUT...
Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
gene stability reference genes quantitative PCR Sus scrofa age categories of pigs muscle tissue
2015/6/9
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each exp...
Carcass and meat quality traits of pig reciprocal crosses with a share of Pietrain breed
pigs reciprocal crosses carcass and meat quality RYR1 genotype
2015/6/9
The study was carried out on 119 hybrid grower-finisher pigs that were divided into 5 experimental groups. The analysis covered carcass and meat quality traits of crossbred pigs produced by mating Pol...
Differential response of Iberian and lean pig crossbreeds to dietary linoleic acid administration
genetic type metabolism swine
2014/10/24
We aimed to determine whether the response of linoleic acid (C18:2 n-6) tissue concentration to dietary C18:2 n-6 administration, in the finishing period, is done in a different way in Iberian pig com...
家猪减数分裂粗线期二价体与有丝分裂中期染色体的比较研究Comparative Studies on Meiosis Pachytene Bivalents and MitosisCMetaphase Chromosomes of Pig (Sus scrofa domestica)
家猪 二价体 有丝分裂染色体 染色粒结构带 G-带
2008/1/30
摘要以性成熟公猪睾丸和外周血为材料，采用长低渗、高氯仿卡诺固定液固定和外周血细胞培养制备减数分裂粗线期二价体和有丝分裂中期染色体，通过对二价体和有丝分裂中期染色体分裂指数和长度的比较研究，发现二价体的分裂指数和长度分别是有丝分裂中期染色体的5倍和3.42倍(1.87～5.98)；同时以12号染色体为例, 比较了二价体上的染色粒结构带与有丝分裂中期染色体G-带，表明染色粒结构带比中期染色体G-带带纹...
摘要应用常规方法获得了杂种猪( 野猪（♂）×家猪（♀）)的核型、C带和174条带纹的G带，应用微量秋水仙素法得到了258条带纹的高分辨G带。结果表明，杂种猪的二倍体细胞染色体数目2n=38，C带具有多态性，G带和高分辨G带与家猪相比无明显差异，它们属于同一种。
Abstract:Using usual method,we got karyotype of hybrid pig (wild soa...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
中外11个猪种A-FABP基因微卫星遗传变异的研究Genetic Variation of A-FABP Microsatellites in Eleven Pig Breeds
猪 A-FABP 基因 微卫星 分化
2008/1/8
摘要
利用微卫星技术对五指山猪、沂蒙黑猪、汉江黑猪、莱芜猪、北京黑猪、民猪、成华猪、内江猪、二花脸猪、巴马香猪和大白猪11个猪种共420头猪的脂肪细胞脂肪酸结合蛋白基因内含子的微卫星序列的遗传变异进行了研究。研究表明：（1）中国猪种在A-FABP基因微卫星基因座上有极高的的多态性，五指山猪的PIC最高（PIC=0.7904）并检测到11个等位基因，但北京黑猪仅检测到两个等位基因。（2）本实验中，...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC)；结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...
猪白细胞Ⅱ类抗原基因多态性研究进展Advances in Allelic Polymorphism at Class Ⅱ of the Pig MHC(SLA)
猪 SLAⅡ类基因 RFLP 多态性
2007/12/31
摘要本文对SLAⅡ类基因的染色体图谱定位、分子结构、基因分型及其多态性的研究进展作了介绍。重点介绍了SLAⅡ类基因的RFLP基因分型及其多态性研究。猪的主要组织相容性复合物Ⅱ类抗原基因座位存在不同程度的等位基因多态性。RFLP技术是一种简便而快捷的SLAⅡ类等位基因分型方法。SLAⅡ类基因的多态性和抗原肽结合位点密切相关，这些位点能结合处理过的抗原，然后递呈给T细胞。另外，在编码β1链的SLA-D...