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Mutation in the RPE65 gene causing hereditary retinal dystrophy in the Briard dogs: application of a new detection method
RPE65 gene CSNB dog Briard electrophoresis
2015/6/5
Inherited eye diseases are widespread in most of the pure dog breeds and they show a severe impact on canine health, welfare and working ability. Congenital stationary night blindness (CSNB) was origi...
Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology
Lawsonia intracellularis nested PCR indirect immunofluorescence
2015/1/28
A nested polymerase chain reaction (PCR) assay and serological examinations were used to detect the presence of Lawsonia intracellularis in a two and a half years old German smooth-coated cocker spani...
Brachyspira hyodysenteriae: detection, identification and antibiotic susceptibility
pigs dysenteries isolation identification Brachyspira hyodysenteriae weakly haemolytic spirochaetes PCR MIC tiamulin tylosin valnemulin
2015/1/26
101 samples of faeces, colonic mucosa and rectal swabs taken from 100 pigs (29 commercial herds) were cultivated on Trypticase Soy Agar with 5% of sheep blood, spectinomycin (200 mg/l), vancomycin (50...
Counter immunoelectrophoresis: a simple method for the detection of species-specific muscle proteins in heat-processed products
counter-immunoelectrophoresis muscle protein thermostable antigen antiserum heat-processed meat products
2015/1/26
Counter immunoelectrophoresis (CIE) was used for the detection of species-specific muscle proteins in food products. This technique allowed the detection of pork, beef, poultry, or and kangaroo meats ...
Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams
Chlamydophila abortus CF test iELISA goats sheep rams
2015/1/26
Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%,...
Comparison of the detection and quantification of rabies antibodies in canine sera
rabies antibodies detection RFFIT FAVN test
2015/1/26
Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neut...
New Screening Test for Male Germline Chimeric Chickens by Polymerase Chain Reaction Using Shingle Nucleotide Polymorphism Detection Primers
chimeric chicken germline mitochondrial DNA D-loop region single nucleotide polymorphism sperm
2009/7/31
Since it is impossible to distinguish germline chimeric chickens by appearance only, a progeny test is required for analyzing germline chimerism. To reduce the labor, time and expense involved in such...
摘要
以绵羊BMP15基因和BMPR-IB基因为候选基因，以湖羊、中国美利奴单胎品系、中国美利奴肉用和毛用多胎品系为研究对象，采用PCR-RFLP方法对候选基因进行单核苷酸多态性（SNP）位点检测和基因型分析，同时研究基因对绵羊产羔数的影响。对BMP15基因进行SNP检测，结果未发现多态性位点；对BMPR-IB基因进行多态性检测，结果发现了一个A746 G SNP位点。依据A746 G SNP位...