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Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA
genetic variability genetic structure conservation microsatellite DNA Kladruber horse line
2015/6/9
The Old Kladruber horse is the oldest original Czech horse breed – gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of...
Genetic diversity of five Chinese goat breeds assessed by microsatellite markers
goat microsatellites biodiversity
2015/6/5
The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). ...
Genetic diversity between seven Central European cattle breeds as revealed by microsatellite analysis
cattle breeds gene resources heterozygosity microsatellites
2015/6/2
This study compares the genetic variation in seven cattle breeds from theterritory ofCentralEurope. Eleven polymorphic microsatellite loci were used to detect differences in the polymorphism of geneti...
Genetic Variability and Relationships of Japanese Native Chickens Assessed by Means of Microsatellite Profiling Approach-Focusing on the Oh-Shamo (Japanese Large Game) and Its Related Breeds
genetic relationship Japanese native chickens microsatellite Shamo (Japanese Game)
2009/7/31
We investigated genetic relationships of nine Japanese native chicken breeds (Oh-Shamo, Ko-Shamo, Yakido, Kinpa, Koeyoshi, Minohiki, Satsuma-dori, Hinai-dori, and Shoukoku) coupled with one foreign br...
Development and Mapping of Microsatellite Markers Derived from cDNA in Japanese Quail (Coturnix japonica)
comparative mapping functional gene Japanese quail linkage map microsatellite
2009/7/31
The objective of this work was to develop polymorphic microsatellite markers derived from Japanese quail embryonic and cardiac cDNA libraries and to locate these markers on Kobe-NIBS Japanese quail (K...
Genetic Variability and Relationships of Native Japanese Chickens Based on Microsatellite DNA Polymorphisms-Focusing on the Natural Monuments of Japan
genetic relationship genetic variability microsatellite DNA polymorphisms native Japanese chickens, Natural Monuments of Japan
2009/7/30
In this experiment, 728 blood samples were collected from 22 breeds (28 populations) of native Japanese chickens that have been designated as Natural Monuments of Japan [Chabo, Gifu-Jidori (2 populati...
Genetic Diversity and Differentiation of the Nagoya Breed Inferred from Microsatellite DNA Polymorphisms
chicken genetic differentiation genetic diversity microsatellites Nagoya breed
2009/7/30
The Nagoya breed is a popular Japanese native chicken, a dual-purpose breed raised for eggs and meat. We used 24 microsatellite markers were employed to analyze the diversity and differentiation of th...
Microsatellite Analysis of Genetic Diversity in Indian Chicken Populations
chicken genetic distance genetic diversity India microsatellite markers
2009/7/30
Characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of poultry genetic resources. We assessed genetic variati...
猪微卫星标记多重PCR扩增组合Amplification of Pig Microsatellite Markers Using Multiplex PCR
猪 微卫星 多重PCR
2008/1/10
摘要采用多重PCR的方法，以快速扩增微卫星标记和节约试剂为目标，对其反应条件进行优化后，获得了46个扩增效果理想的微卫星标记多重PCR组合，其中30个为二重PCR，16个为三重PCR。实验结果表明，这些多重PCP反应的引物浓度为0.06~0.3μmol/L， Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6，每个PCR反应聚合酶的用量在0...
不同猪种肠毒素大肠杆菌（ETEC）F4受体微卫星标记遗传差异性研究The Genetic Variation of Two Microsatellite Markers of Escherichia coli F4(K88) Receptor in Different Swine Breeds
ETEC 微卫星 基因杂合度 Shannon信息指数 遗传标记
2008/1/10
摘要采用13号染色体上与K88ab和K88ac受体基因连锁的2对引物（S0223和S0068），研究沙子岭猪和大约克猪的遗传差异性。结果表明，2个猪种在2个基因座均存在多态性，其基因杂合度和Shannon信息指数存在很大差异，而中外猪种的K88ab和K88ac受体基因也存在遗传差异，这2对引物可望作为K88ab和K88ac受体基因的遗传标记。Abstract: The genetic variat...
利用微卫星标记进行凉山半细毛羊亲权鉴定的研究Microsatellite Markers for Paternity Testing of Liangshan Semi-fine Wool Sheep
凉山半细毛羊 微卫星标记 多重PCR 亲权鉴定
2008/1/6
摘要
经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物，并用Cervus 2.0分析。结果表明：当双亲未知时，累积排除概率为0.998666 ，在父系半同胞基础上进行母子亲权鉴定，累积排除概率为0.999994，置信度为95%，最终从8个候选父亲中找出215个后裔的父亲，又从147个候选母亲中找出它们的母亲，构建了一个混合家系系谱，为进行连锁分析、QTL定位...
应用多重PCR和基因扫描技术对五指山猪13个家系32个微卫星基因座的遗传分析Genetic Analysis of 32 Microsatellite Loci in 13 Families of Wuzhishan Pig by Multiplex PCR and Gene scaning Technique
五指山猪 微卫星 基因扫描 遗传多样性
2008/1/6
摘要
五指山猪是我国著名的珍稀畜种,体型小、性成熟早、遗传稳定、近交程度高,可用于医学、药学和生物材料等方面。作者利用多重PCR和基因扫描技术对五指山猪13个家系的32个微卫星基因座进行遗传检测。统计各家系的等位基因组成,计算各家系的平均杂合度和多态信息含量(PIC)；结果,各家系32基因座的平均等位基因数为13.66个,平均PIC为0.731,平均杂合度为0.559。表明海南五指山猪具有丰富的...