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De novo phasing resolves haplotype sequences in complex plant genomes
de novo phasing haplotype-resolved assembly autopolyploid chromosomal rearrangement
2023/11/28
Genome phasing is a recently developed assembly method that separates heterozygous eukaryotic genomic regions and builds haplotype-resolved assemblies. Because differences between haplotypes are ignor...
Engineering the plastid and mitochondrial genomes of flowering plants
flowering plants plastid genome mitochondrial genome CRISPR/Cas9 Engineering transcription activator-like genome editing
2023/11/16
Engineering the plastid genome based on homologous recombination is well developed in a few model species. Homologous recombination is also the rule in mitochondria, but transformation of the mitochon...
北京市农林科学院林业果树研究所与多国合作编著出版 《Underutilised Crop Genomes》（图）
杏 种质资源 林业果树研究所 《Underutilised Crop Genomes》
2023/2/27
北京市农林科学院林业果树研究所杏李研究室与多国专家合作编写《Underutilised Crop Genomes》一书，于2022年11月上线，网址DOI https://doi.org/10.1007/978-3-031-00848-1。
Genomes of 5 cotton species could lead to better varieties（图）
Genomes 5 cotton species better varieties
2020/4/29
Cotton producers in the U.S. and around the world are looking for new varieties that can better withstand droughts, pests, and pathogens while yielding high-quality fibers for the textile industry.To ...
Genome-wide analysis of CpG islands in some livestock genomes and their relationship with genomic features
cattle gene networks GO GWAS KEGG mixed model SNP validation
2016/11/11
When analyzing phenotypes undergoing a complex mode of inheritance, it is of great interest to switch the scope from single genes to gene pathways, which form better defined functional units. We used ...
New Tools for Discovering DNA Variation in Crop Genomes
New Tools DNA Variation Crop Genomes
2009/12/16
The study of human genetics has been a successful venture for researchers in recent years. Several million single-nucleotide polymorphisms (SNPs) have been identified from the whole-genome resequenci...
摘要用碱处理水稻嫩叶，取碱处理水稻叶片浸出液直接用作PCR扩增的模板，扩增结果稳定、准确，与常规提取的DNA扩增效果相比没有显著差异。用此方法制备的模板室温下2周之内、4℃下3周之内、-20℃下4个月以上扩增结果不变。此方法所需试剂均为常规试剂，具有需要材料少、成本低、简便、快捷等优点，尤其适合材料比较宝贵的转基因水稻的预筛选和大样本量的PCR检测，为分子标记技术的实际应用创造了条件。
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