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Surfactin – A Review on Biosynthesis, Fermentation, Purification and Applications
biosurfactant surfactin Bacillus subtilis biosynthesis fermentation purification
2010/10/14
Surfactin, a bacterial cyclic lipopeptide, is produced by various strains of Bacillus subtilis
and is primarily recognized as one of the most effective biosurfactants. It has the ability to
reduce s...
Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment
Antagonistic activity bacteriocin indicator organisms lactic acid bacteria
2010/9/30
Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production...
Purification and Gene Cloning of Catalase from Staphylococcus warneri ISK-1
catalase Staphylococcus purification
2009/7/8
To investigate the possibility of applying staphylococcal catalase to eliminate H2O2 in lactic acid fermentation, an intracellular catalase from Staphylococcus warneri ISK-1 which exhibited high catal...
Purification and Characterization of Extracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/8
An extracellular cysteine protease inhibitor (ECPI) from Chlorella sp. was purified to homogeneity by DEAE-Cellulose column chromatography and Sephacryl S-300 column chromatography. The molecular mass...
Purification and Some Properties of Pectinesterase from Fruits of a Miniature-Fruited Red Type Tomato
pectinesterase tomato fruits affinity chromatography
2009/7/8
A pectinesterase (E.C. 3.1.1.11, pectin pectylhydrolase) from the ripening stage of fruits of a miniature-fruited red type tomato (Mini-tomato) was purified 247 fold with yield of 26% by ammonium sulf...
Purification and Characterization of Phospholipase D from Cabbage Leaves
phospholipase D cabbage N-terminal amino acid sequence
2009/7/8
Phospholipase D (PLD) was purified from cabbage leaves. The molecular weight of purified PLD was estimated as approximately 73 and 87 kDa by gel filtration using Superdex 200 HR column and, sodium dod...
Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
Aspergillus awamori feruloyl carbohydrate ester feruloyl esterase
2009/7/7
A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatograph...
Purification of Endopeptidase in Yam Dioscorea opposita and Its Characterization
yam endopeptidase purification
2009/7/7
An endopeptidase in powdered yam was purified using a combination of anion-exchange and gel-filtration chromatographies. The preparation gave 9.2-fold purification with yield of 19% against the crude ...
Purification and Characterization of Intracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/7
An intracellular cysteine protease inhibitor (ICPI) from Chlorella sp. was purified to homogeneity judging from polyacrylamide gel electrophoresis. The purified inhibitor had a molecular mass of 410kD...
Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
phytase phytic acid wheat bran
2009/7/7
Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration ...
Pilot-Scale Study of the Purification and Concentration of Non-Digestible Saccharides from Yacon Rootstock Using Membrane Technology
membrane separation oligosaccharides purification food processing
2009/6/17
The effectiveness of a membrane process combining ultrafiltration (UF) and nanofiltration (NF) for purifying and concentrating non-digestible saccharides (NDS) from yacon rootstock was examined. The y...
Purification and Characterization of Glutamate Decarboxylase from Aspergillus oryzae
glutamate decarboxylase Aspergillus oryzae purification
2009/6/17
We purified glutamate decarboxylase (GAD) [EC4.1.1.15] from Aspergillus oryzae and characterized its biochemical and kinetic properties. GAD was purified by ammonium sulfate at 30–70% saturation and c...
Purification and Characterization of Wheat（Triticum aestivum）Polyphenol Oxidase
wheat polyphenol oxidase purification
2009/6/10
Polyphenol oxidase was purified to homogeneity from Japanese wheat (Triticum aestivum cv. Tohoku 198) bran, using 4-methylcatechol as a substrate. The enzyme was purified 1670-fold with a recovery of ...
Purification and Characterization of an Extracellular Proteinase Having Milk-Clotting Activity from Enterococcus faecalis TUA2495L
milk-clotting enzyme extracellular proteinase Enterococcus faecalis
2009/5/31
Lactic acid bacteria (157 stock cultures) were screened for their ability to produce extracellular proteinase with milk-clotting activity. A strain identified as Enterococcus faecalis TUA2495L showed ...
Purification and Characterization of Polyphenol Oxidase from Edible Yam（Dioscorea opposita Thunb.）
polyphenol oxidase edible yam dopamine oxidase
2009/5/31
Soluble polyphenol oxidase of edible yam (Dioscorea opposita Thunb.) was purified approximately 203-fold with a recovery rate of 15% by ammonium sulfate fractionation, ion exchange chromatography, hyd...