搜索结果: 1-12 共查到“食品生物化学 PCR”相关记录12条 . 查询时间(0.132 秒)
Food safety and label claims for hazelnut allergy traces:evaluation of two PCR assays
amplification declaration food allergy hazelnut
2015/10/9
The molecular techniques (C-PCR, RT-PCR) in the detection and quantification of allergic substances of hazelnut in various categories of food commodities, e.g. breakfast cereals, chocolates and biscui...
实时荧光定量PCR法与分离培养法检测食品从业人员沙门菌和志贺菌的比较研究
分离培养法 比较 食源性致病菌
2015/11/17
比较实时荧光定量PCR法(RT-PCR)与分离培养法对食品从业人员肠道致病菌沙门菌和志贺菌的检测效果。方法 ①RT-PCR法:用实时荧光定量PCR对体检肛拭子进行初筛；②分离培养法:按照国标方法GB/T 4789.4—2010《食品安全国家标准 食品微生物学检验 沙门氏菌检验》和GB/T 4789.5—2003《食品卫生微生物学检验 志贺氏菌检验》对所筛选出的菌株进行鉴定。结果 RT-PCR法对食...
荧光定量PCR与常规PCR法检测GⅡ型札幌样病毒的比较
GⅡ型札幌样病毒 荧光定量PCR 常规RT-PCR
2015/12/10
目的比较荧光定量PCR与常规RT-PCR法检测牡蛎中GⅡ型札幌样病毒的灵敏度与准确性,为该病毒检测提供适用的方法。方法采用荧光定量PCR和RT-PCR方法,分别检测牡蛎样品中的GⅡ型札幌样病毒。结果荧光定量PCR检测的灵敏度可达102拷贝/μl,札幌样病毒呈阳性的牡蛎有6例,阳性率为4.72%(6/127);RT-PCR的灵敏度为103拷贝/μl,札幌样病毒呈阳性的牡蛎有1例,阳性率为0.79%(...
Development and verification of PCR based assay to dectect and quantify garden pea lec gene
GMO lectin PCR detection real-time PCR
2014/2/25
Genetically modified organisms (GMOs) entering the food chain have became its part, which is necessary to monitor. GMO analyses are used as a control mechanism according to valid acquis communautaire ...
Reliability of PCR based screening for identification and quantification of GMOs
GMO (Genetically Modified Organism) identification PCR real-time PCR screening RR soya
2014/2/27
Handling with genetically modified organisms (GMOs) is regulated namely in EC. Laboratories often use polymerase chain reaction (PCR) based screening methods to monitor the presence of GM particles in...
Differentiation of Bacillus cereus Isolates from Milk and Milk Products with Biochemical, Immunological,AP-PCR and PCR-RFLP Methods
Bacillus cereus milk genotyping
2009/3/23
Physiological features including lecithinase and haemolytic activity, API biotyping and immunodetection of diarrhoeal enterotoxin were compared with AP-PCR genotyping and PCR-RFLP analysis of hblA and...
Polymorphism of κ -Casein in Italian Goat Breeds:A New ACRS-PCR Designed DNA Test for Discrimination of A and B Alleles
ACRS-PCR genetic polymorphism goat κ-casein
2009/3/20
The objective of this study was to develop a DNA test for rapid characterisation of goat κ-casein (κ-CN) A and B variants and to study this polymorphism in Italian goat breeds. Genetic polymorphism of...
Genotypic Characterisation of Indigenous Soybean Rhizobia by PCR-RFLP of 16S rDNA,rep-PCR and RAPD Analysis
nitrogen fixation rhizobia soybean
2009/3/19
The taxonomy of nitrogen fixing bacteria that form symbiotic associations with leguminous plants has been deeply changed in recent years. The use of very sensitive and accurate molecular methods has e...
Evaluation of Diagnostic PCR for the Detection of Listeria monocytogenes in Food Products
Listeria monocytogenes PCR diagnostic food products
2009/3/16
Conventional methods for the detection of Listeria in foodstuffs are generally cumbersome and time consuming. The use of primary enrichment in ½ strength Fraser broth and the use of Oxford and ...
Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA
aminopeptidase N APN competitive PCR
2009/3/16
Comparison of three RT-PCR based methods: semi-quantitative, competitive and real-time RT-PCR for relative quantification of mRNA is presented. Aminopeptidase N expressed on human promyeloid HL-60 cel...
Interferences of PCR effectivity: importance for quantitative analyse
real-time PCR DNA quantification PCR efficiency GMO analysis
2014/3/10
Importance of the Polymerase chain reaction (PCR) have already crossed the border of mere target DNA sequence present or absence analysis. For number analyses e.g. Genetically Modified Organisms (GMOs...