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Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Porcine Oocyte Antioxidants Quercetin Reactive Oxygen Species [ROS]
2016/5/19
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate ...
The role of nitric oxide in parthenogenetic activation of pig oocytes: A review
oocyte parthenogenesis activation nitric oxide
2015/6/4
Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies fo...
Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor
oocyte pig parthenogenesis activation calcium ionophore nitric oxide
2015/6/4
Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC)...
Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
Brilliant Cresyl Blue Embryonic Development Subcellular Characteristics
2016/5/19
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogena...
Rapamycin Rescues the Poor Developmental Capacity of Aged Porcine Oocytes
Reactive Oxygen Species (ROS) Mammalian Target of Rapamycin (mTOR)
2016/5/16
Unfertilized oocytes age inevitably after ovulation, which limits their fertilizable life span and embryonic development. Rapamycin affects mammalian target of rapamycin (mTOR) expression and cytoskel...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Growth of Ovarian Primary Follicles Retrieved from Neonates of Different Ages and Derivation of Mature Oocytes Following In vitro-Culture
Neonatal Mouse Ovary Primary Follicle In vitro Culture Oocyte Maturation
2016/5/10
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follic...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine
Suppression Subtractive Hybridization (SSH) Gene Expression Cumulus Cells Porcine
2016/5/3
In the antral follicle phase, several layers of cumulus cells surround the oocyte and play an important support and regulation role in oocyte development and maturation via intercellular communication...
Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits
Nuclear Transfer Oocyte Rabbit Follicle Somatic Cell
2016/5/3
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients ...
Bovine Oocytes Can Be Penetrated in Modified Tris-buffered Medium
Bovine In vitro Fertilization Tris-buffer Glucose In vitro Culture
2016/4/29
A modified Tris-buffered medium (mTBM) has been widely used as an insemination medium for porcine in vitro fertilization (IVF). We examined whether mTBM could be used for bovine IVF. Bovine cumulus-oo...
The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes
Cryoprotectant Porcine Oocytes ATP Content
2016/4/29
This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs)...
Stage-specific Expression of Lanosterol 14-Demethylase in Mouse Oocytes in Relation to Fertilization and Embryo Development Competence
Mouse Oocyte Lanosterol 14 -Demethylase In vitro Fertilization Early Embryo Development
2016/4/29
Follicular fluid meiosis-activating sterol (FF-MAS) has been suggested as a positive factor which could improve the oocyte quality and subsequent embryo development after in vitro fertilization. Howev...
Effects of β-Mercaptoethanol on the Growth of Preantral Follicles and the Maturation of Intrafollicular Oocytes
Preantral Follicle In-vitro Culture b-Mercaptoethanol Follicle Growth Oocyte Maturation
2016/4/28
This study was undertaken to evaluate how -mercaptoethanol (bME), an exogenous antioxidant, interacts with preantral follicles cultured in vitro. Mouse primary or secondary follicles were cult...
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Murine Bovine Interspecies Somatic Cell Nuclear Transfer b-Mercaptoethanol Xist
2016/4/28
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studie...
Association between HSP70 Genotypes and Oocytes Development on In vitro Maturation/Fertilization in Pig
Fertilization Maturation Porcine Oocytes HSP70 Genotypes
2016/4/28
This study was performed to clarify whether the variation of stress related heat shock protein 70 (HSP70) (GenBank X68213) gene was associated with the nuclear morphological change of in vitro maturat...