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General-use polymerase chain reaction primers for amplification and direct sequencing of enolase, a single-copy nuclear gene, from different animal phyla
enolase Metazoa, scnDNA
2015/12/11
The dearth of general-use (i.e. quasi-‘universal’) primer
sets for the amplification of single-copy nuclear DNA
(scnDNA) loci, combined with the availability and ease of
use of mitochondrial (mtDNA...
16S～23S rDNA间区在链球菌和流感嗜血杆菌分类中的应用Identification of Streptococcus Species and Haemophilus influenzae by Direct Sequencing of PCR Products from 16S~23S rRNA Itergenic Sacer Rgions
16S~23Sr RNA间区 链球菌 流感嗜血杆菌 分类
2007/12/30
摘要利用16S~23S rDNA间区（intergenic spacer regions,ISR）在不同细菌中拷贝数、碱基排列、序列长度及所含tRNA基因种类和数目的差异，对15株链球菌和流感嗜血杆菌进行属、种、型和株系的分类鉴定。在16S rDNA的3′端和23S rDNA的5′端的保守区中合成引物，PCR扩增16S~23S rDNA ISR序列，对多态片段切胶纯化直接测序。在GenBank上查...